caa a22 4500 445823216 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10616-011-9353-x doi (NATIONALLICENCE)springer-10.1007/s10616-011-9353-x Spontaneous differentiation of porcine neural progenitors in vitro [Elektronische Daten] [Fei Yin, Li Guo, Ri-feng Lu, Qing-san Zhu] The pig is the non-primate species that is immunologically closest to humans, and has been considered as an alternative source to human allografts for transplantation. In fact, there has been recent interest in identifying and culturing porcine neural progenitor cells (PNPCs) in vitro, but the long-term culturing has not yet been characterized. Here, we reported the spontaneous differentiation of PNPCs into neuronal and glial cells. For in vitro cultures, the primary cells of the subventricular zone of the forebrain striatum were cultured in the presence of epidermal growth factor and basic fibroblast growth factor to allow the growth of spherical masses that exhibit sustained growth and self-renewal capacity. After growth factor removal, the neurospheres with 10 and 130days of culture spontaneously differentiated into Tuj1-positive neurons and GFAP-positive astrocytes as seen by double immunocytofluorescence. Molecular characterization using reverse transcription-polymerase chain reaction showed that neurospheres expressed nestin, neuron-specific enolase, and glial fibrillary acidic protein (GFAP). In addition, after cultured in the differentiation medium for 3months, the growth of neurosphere became slow and displayed cystic structures with the same morphology as that of embryonic bodies derived from embryonic stem cells. It is concluded that PNPCs have the ability to provide an expandable source of neural cells that can develop into neuronal and glial subtypes. Springer Science+Business Media B.V., 2011 Porcine nationallicence Neural progenitors nationallicence Differentiation nationallicence Neurons nationallicence Astrocytes nationallicence Yin Fei Department of Spine Surgery, The First Hospital, Jilin University, 130021, Changchun, China aut Guo Li Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Street, 130021, Chanchun, Jilin, People's Republic of China aut Lu Ri-feng Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Street, 130021, Chanchun, Jilin, People's Republic of China aut Zhu Qing-san Department of Spine Surgery, The First Hospital, Jilin University, 130021, Changchun, China aut Cytotechnology Springer Netherlands 63/4(2011-08-01), 363-370 0920-9069 63:4<363 2011 63 10616 https://doi.org/10.1007/s10616-011-9353-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9353-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yin Fei Department of Spine Surgery, The First Hospital, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Guo Li Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Street, 130021, Chanchun, Jilin, People's Republic of China aut NATIONALLICENCE 700 1- Lu Ri-feng Department of Toxicology, School of Public Health, Jilin University, 1163 Xinmin Street, 130021, Chanchun, Jilin, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Qing-san Department of Spine Surgery, The First Hospital, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/4(2011-08-01), 363-370 0920-9069 63:4<363 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer