caa a22 4500 445823224 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10616-011-9362-9 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9362-9 Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization [Elektronische Daten] [Marisa Geens, Theo Niewold] The high similarity between pigs and humans makes pigs a good gastrointestinal (GI) model for humans. Recently an epithelial cell line originating from the jejunum of pig (IPEC-J2) became available. Once validated, this model can be used to investigate the complex interactions occurring in the intestine. The advantages of using IPEC-J2 as in vitro model of the GI tract are the high resemblance between humans and pigs, and the ease of extrapolating in vitro to in vivo characteristics. In this study, the IPEC-J2 cells were functionally characterized by measuring the trans-epithelial electrical resistance (TEER), and by histological and ultrastructural studies. IPEC-J2 cells grown on six different permeable support systems, were investigated. The Transwell®-COL collagen-coated membrane (1.12cm2) showed the best results concerning time efficiency and TEER values. The optimum seeding density of 12×105cells/mL ensured that after 9days of differentiation a confluent monolayer was formed. The decrease in TEER values after a maximum had been reached, coincided with the ultrastructural development of apical microvilli. We conclude that IPEC-J2 cells grown on collagen-coated membranes represent a valuable in vitro model system for the small intestinal epithelium which can be of great interest for intestinal research. Springer Science+Business Media B.V., 2011 Pig nationallicence IPEC-J2 nationallicence Boyden chamber nationallicence Model system nationallicence Geens Marisa Division of Livestock-Nutrition-Quality, Department of Biosystems, K.U. Leuven, Kasteelpark Arenberg 30—bus 2456, 3001, Heverlee, Belgium aut Niewold Theo Division of Livestock-Nutrition-Quality, Department of Biosystems, K.U. Leuven, Kasteelpark Arenberg 30—bus 2456, 3001, Heverlee, Belgium aut Cytotechnology Springer Netherlands 63/4(2011-08-01), 415-423 0920-9069 63:4<415 2011 63 10616 https://doi.org/10.1007/s10616-011-9362-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9362-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Geens Marisa Division of Livestock-Nutrition-Quality, Department of Biosystems, K.U. Leuven, Kasteelpark Arenberg 30—bus 2456, 3001, Heverlee, Belgium aut NATIONALLICENCE 700 1- Niewold Theo Division of Livestock-Nutrition-Quality, Department of Biosystems, K.U. Leuven, Kasteelpark Arenberg 30—bus 2456, 3001, Heverlee, Belgium aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/4(2011-08-01), 415-423 0920-9069 63:4<415 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer