caa a22 4500 445823240 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10616-011-9361-x doi (NATIONALLICENCE)springer-10.1007/s10616-011-9361-x On-line monitoring of oxygen in Tubespin, a novel, small-scale disposable bioreactor [Elektronische Daten] [Kui Xie, Xiao-Wei Zhang, Li Huang, Yi-Ting Wang, Yun Lei, Jing Rong, Chui-Wen Qian, Qiu-Ling Xie, Yi-Fei Wang, An Hong, Sheng Xiong] A novel, optical sensor was fixed in a new type of disposable bioreactor, Tubespin, for the on-line (real-time) monitoring of dissolved oxygen concentrations during cell culture. The cell density, viability and volumetric mass transfer coefficient were also determined to further characterize the bioreactors. The kLa value of the Tubespin at standard conditions was 24.3h−1, while that of a spinner flask was only 2.7h−1. The maximum cell density in the Tubespin bioreactor reached 6×106 cellsmL−1, which was two times higher than the cell density in a spinner flask. Furthermore, the dynamic dissolved oxygen level was maintained above 90% air-saturation in the Tubespin, while the value was only 1.9% in a spinner flask. These results demonstrate the competitive advantage of using the Tubespin system over spinner flasks for process optimization and scale-down studies of oxygen transfer and cell growth. Springer Science+Business Media B.V., 2011 Tubespin nationallicence Spinner flask nationallicence Dissolved oxygen nationallicence kLa nationallicence Xie Kui Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Zhang Xiao-Wei Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Huang Li Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Wang Yi-Ting Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Lei Yun Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Rong Jing Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Qian Chui-Wen Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Xie Qiu-Ling Institute of Bioengineering, Jinan University, 510632, Guangzhou, Guangdong, People's Republic of China aut Wang Yi-Fei Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Hong An Institute of Bioengineering, Jinan University, 510632, Guangzhou, Guangdong, People's Republic of China aut Xiong Sheng Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut Cytotechnology Springer Netherlands 63/4(2011-08-01), 345-350 0920-9069 63:4<345 2011 63 10616 https://doi.org/10.1007/s10616-011-9361-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9361-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xie Kui Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xiao-Wei Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Huang Li Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yi-Ting Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Lei Yun Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Rong Jing Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Qian Chui-Wen Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Xie Qiu-Ling Institute of Bioengineering, Jinan University, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yi-Fei Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Hong An Institute of Bioengineering, Jinan University, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 700 1- Xiong Sheng Biomedical R&D Center, Guangdong Provincial Key Laboratory of Bioengineering Medicine, National Engineering Research Center of Genetic Medicine, Jinan University, 5/F Building of Biology, 510632, Guangzhou, Guangdong, People's Republic of China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/4(2011-08-01), 345-350 0920-9069 63:4<345 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer