caa a22 4500 445823283 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10616-011-9363-8 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9363-8 Anticancer effects of 6- o -palmitoyl-ascorbate combined with a capacitive-resistive electric transfer hyperthermic apparatus as compared with ascorbate in relation to ascorbyl radical generation [Elektronische Daten] [Shinya Kato, Ryoko Asada, Katsuhiro Kageyama, Yasukazu Saitoh, Nobuhiko Miwa] The aim of the present study is to determine the anti-proliferative activity of 6-o-palmitoyl-l-ascorbic acid (Asc6Palm) that is a lipophilic derivative of l-ascorbic acid (Asc), on human tongue squamous carcinoma HSC-4 cells by combined use of hyperthermia in comparison to Asc. Asc6Palm or Asc were administered to HSC-4 cells for 1h, to which hyperthermia at 42°C was applied for initial 15min. After further 1-72h incubation at 37°C, cell proliferation was determined with Crystal Violet staining. Ascorbyl radical (AscR) in HSC-4 cell suspension was measured by electron spin resonance (ESR), and cell morphology was observed with scanning electron microscopy (SEM). At 37°C, 4mM Asc or 0.35mM Asc6Palm were enough to suppress proliferation of HSC-4 cells. By combined use of hyperthermia at 42°C, cell proliferation was decreased when compared to 37°C. After Asc of 4mM was incubated with HSC-4 cell suspensions at 37°C or 42°C for 0-180min, the signal intensity of ascorbyl radical (AscR) by ESR was not different regardless of the presence or absence of cells at 37°C, whereas AscR signal was enlarged in the presence of HSC-4 cells at 42°C. It was suggested that oxidation of Asc occurred rapidly in HSC-4 cells by hyperthermia, and thereby enhanced the anti-proliferative activity. By SEM observation, the surface of HSC-4 cells treated with Asc6Palm revealed distinct morphological changes. Thus, the combined regimen of Asc6Palm and hyperthermia is expected to exert a marked antitumor activity. Springer Science+Business Media B.V., 2011 6- o -Palmitoyl- l -ascorbic acid nationallicence Hyperthermia nationallicence Human tongue squamous carcinoma cells HSC-4 nationallicence Crystal Violet staining nationallicence Electron spin resonance (ESR) nationallicence Scanning electron microscopy (SEM) nationallicence Asc6Palm : 6-o-palmitoyl-l-ascorbic acid nationallicence Asc : l-ascorbic acid nationallicence AscR : Ascorbyl radical nationallicence ESR : Electron spin resonance nationallicence SEM : Scanning electron microscopy nationallicence CRet system : Capacitive-resistive transfer system nationallicence Kato Shinya Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, 727-0023, Hiroshima, Japan aut Asada Ryoko Osaka Butsuryo College, OtoriHigashiMachi 4-410-5, Nishi-ku, Sakai, 593-8324, Osaka, Japan aut Kageyama Katsuhiro Osaka Butsuryo College, OtoriHigashiMachi 4-410-5, Nishi-ku, Sakai, 593-8324, Osaka, Japan aut Saitoh Yasukazu Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, 727-0023, Hiroshima, Japan aut Miwa Nobuhiko Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, 727-0023, Hiroshima, Japan aut Cytotechnology Springer Netherlands 63/4(2011-08-01), 425-435 0920-9069 63:4<425 2011 63 10616 https://doi.org/10.1007/s10616-011-9363-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9363-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato Shinya Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, 727-0023, Hiroshima, Japan aut NATIONALLICENCE 700 1- Asada Ryoko Osaka Butsuryo College, OtoriHigashiMachi 4-410-5, Nishi-ku, Sakai, 593-8324, Osaka, Japan aut NATIONALLICENCE 700 1- Kageyama Katsuhiro Osaka Butsuryo College, OtoriHigashiMachi 4-410-5, Nishi-ku, Sakai, 593-8324, Osaka, Japan aut NATIONALLICENCE 700 1- Saitoh Yasukazu Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, 727-0023, Hiroshima, Japan aut NATIONALLICENCE 700 1- Miwa Nobuhiko Laboratory of Cell-Death Control BioTechnology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, 727-0023, Hiroshima, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/4(2011-08-01), 425-435 0920-9069 63:4<425 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer