caa a22 4500 445823291 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10616-011-9355-8 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9355-8 Using 96-well tissue culture polystyrene plates and a fluorescence plate reader as tools to study the survival and inactivation of viruses on surfaces [Elektronische Daten] [Phuc Pham, Junwon Jung, Niels Bols] A method for studying the behavior of viruses on surfaces has been developed and is illustrated by determining the temperatures that inactivate adsorbed viral hemorrhagic septicemia virus (VHSV) and the concentration of 1-propanol that disinfected surfaces with adsorbed VHSV and chum salmon virus (CSV). VHSV is a rhabdovirus; CSV, a reovirus, and they were detected with two fish cell lines, EPC and CHSE-214, respectively. When polystyrene tissue culture surfaces were incubated with virus, rinsed, and left to dry, they still supported the attachment and spreading of cell lines and after 7days these cells showed the characteristic CPE of the viruses. Thus cells appeared to be infected directly from surfaces on which viruses had been adsorbed. Applying this property to 96-well plates allowed duplicate surfaces to be examined for their infectiousness or support of CPE. For each treatment 80 replicate surfaces in a 96-well plate were tested at one time and the results expressed as the number of wells showing CPE. VHSV adsorbed to polystyrene was inactivated by drying in the dark at temperatures above 14°C, but remained infectious for at least 15days of drying at 4°C. For chemical sterilization of polystyrene surfaces with adsorbed virus, disinfection was achieved with 1-propanol at 40% for VHSV and at 60% for CSV. As CPE can be conveniently monitored in 96-well plates with a fluorescence plate reader, this method can be used to rapidly evaluate a variety of treatments for their ability to inactivate surface-bound viruses. Springer Science+Business Media B.V., 2011 Viral adsorption nationallicence Surface disinfection nationallicence Viral inactivation nationallicence Fomites nationallicence Fish nationallicence VHSV nationallicence CSV nationallicence Pham Phuc Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada aut Jung Junwon Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada aut Bols Niels Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada aut Cytotechnology Springer Netherlands 63/4(2011-08-01), 385-397 0920-9069 63:4<385 2011 63 10616 https://doi.org/10.1007/s10616-011-9355-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9355-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pham Phuc Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada aut NATIONALLICENCE 700 1- Jung Junwon Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada aut NATIONALLICENCE 700 1- Bols Niels Department of Biology, University of Waterloo, N2L 3G1, Waterloo, ON, Canada aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/4(2011-08-01), 385-397 0920-9069 63:4<385 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer