caa a22 4500 445823305 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9374-5 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9374-5 Human adipose tissue derived mesenchymal stem cells are resistant to several chemotherapeutic agents [Elektronische Daten] [Wei Liang, Hailong Xia, Jing Li, Robert Zhao] Human adipose derived mesenchymal stem cells (ADMSCs) are multipotential stem cells, originated from the vascular stromal compartment of fat tissues which can be used as an alternative cell source for many different cell therapies. However, their response to chemotherapeutic agants remains unknown. Here we assessed the acute direct effects of individual chemotherapeutic drug on ADMSCs. Using an in vitro culture system, the response of ADMSCs to the three chemotherapeutic agents cisplatin, comptothecin and vincristine was determined in comparison with that of testicular germ cell tumour (TGCT) cell line. The recovery of cell numbers following exposure to chemotherapeutic agents were also evaluated. Our results showed that human ADMSCs were resistant to chemo-therapeutic agents which are commonly used in clinic, the full recovery was seen respectively in ADMSCs after the drug treatment. Moreover, ADMSCs maintained their stem cell characteristics in vitro after the exposure to all chemotherapeutic agents. Springer Science+Business Media B.V., 2011 Adipose tissue nationallicence MSCs nationallicence Chemoresistance nationallicence Cisplatin nationallicence Vincristine nationallicence Camptothecin nationallicence ADMSC : Adipose derived mesenchymal stem cell nationallicence MSCs : Mesenchymal stem cells nationallicence XTT : 2,3-Bis(2-methoxy-4-nitro-5-sulphophenyl)-5-((phenylamino)carbonyl)2H-tetrazolium hydroxide nationallicence FITC : Fluoresein-5-isothiocyanate nationallicence FACS : Fluorescence-activated cell sorting nationallicence Liang Wei Department of Oncology, Center for Cell Therapy and Tissue Engineering, The First Affiliated Hospital of Anhui Medical University, 218# Jixi Road, 230022, Hefei, Anhui Province, People's Republic of China aut Xia Hailong Department of Oncology, Center for Cell Therapy and Tissue Engineering, The First Affiliated Hospital of Anhui Medical University, 218# Jixi Road, 230022, Hefei, Anhui Province, People's Republic of China aut Li Jing Institute of Basic Medical Science and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People's Republic of China aut Zhao Robert Institute of Basic Medical Science and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People's Republic of China aut Cytotechnology Springer Netherlands 63/5(2011-10-01), 523-530 0920-9069 63:5<523 2011 63 10616 https://doi.org/10.1007/s10616-011-9374-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9374-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liang Wei Department of Oncology, Center for Cell Therapy and Tissue Engineering, The First Affiliated Hospital of Anhui Medical University, 218# Jixi Road, 230022, Hefei, Anhui Province, People's Republic of China aut NATIONALLICENCE 700 1- Xia Hailong Department of Oncology, Center for Cell Therapy and Tissue Engineering, The First Affiliated Hospital of Anhui Medical University, 218# Jixi Road, 230022, Hefei, Anhui Province, People's Republic of China aut NATIONALLICENCE 700 1- Li Jing Institute of Basic Medical Science and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Robert Institute of Basic Medical Science and School of Basic Medicine, Center of Excellence in Tissue Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 523-530 0920-9069 63:5<523 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer