caa a22 4500 445823313 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9376-3 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9376-3 Bone marrow mesenchymal stem cells differentiate into urothelial cells and the implications for reconstructing urinary bladder mucosa [Elektronische Daten] [Jiwei Ning, Changying Li, Hongjie Li, Jiwu Chang] To determine the ability of cultured bone marrow-derived mesenchymal stem cells (BMSCs) to differentiate into functional urothelium. BMSCs were isolated from the long bones of aborted fetal limbs by Percoll density gradient centrifugation and characterized by flow cytometry. Human fetal urinary bladders were cut into small pieces and cultured for 3-5days until the growth of urothelial cells was established. BMSCs were then cocultured with neonatal urothelial cells and subsequently evaluated for antigen expression and ultramicrostructure, by immunocytochemistry and electron microscopy, respectively. A subset of BMSCs expressed the differentiation marker CD71. The BMSC markers CD34, CD45, and HLA-DR were barely detectable, confirming that these cells were not derived from hematopoietic stem cells or differentiated cells. In contrast, the stem cell markers CD29, CD44, CD105, and CD90 were highly expressed. BMSCs possessed the ability to differentiate into a variety of cellular subtypes, including osteocytes, adipocytes, and chondrocytes. The shapes of BMSCs changed, and the size of the cells increased, following in vitro coculture with urothelial cells. After 2weeks of coculture, immunostaining of the newly differentiated BMSCs positively displayed the urothelial-specific keratin marker. Electron microscopy revealed that the cocultured BMSCs had microstructural features characteristic of epithelial cells. Pluripotent BMSCs can transdifferentiate into urothelial cells in response to an environment conditioned by neonatal urothelial cells, providing a means for the time-, labor- and cost-effective reconstruction of urinary bladder mucosa. Springer Science+Business Media B.V., 2011 Urothelial cells nationallicence Stem cells nationallicence Differentiation nationallicence Coculture nationallicence Ning Jiwei Research Laboratory for Tumor Immunity, Tianjin Urological Surgery Institute, Second Affiliated Hospital, Tianjin Medical University, 23 Pingjiang Road, 300211, Hexi District, Tianjin, China aut Li Changying Research Laboratory for Tumor Immunity, Tianjin Urological Surgery Institute, Second Affiliated Hospital, Tianjin Medical University, 23 Pingjiang Road, 300211, Hexi District, Tianjin, China aut Li Hongjie Department of Pathophysiology, Institute of Basic Medicine, HeBei United University, 57 Jianshe Road, 063000, Lubei District, Tangshan, China aut Chang Jiwu Research Laboratory for Tumor Immunity, Tianjin Urological Surgery Institute, Second Affiliated Hospital, Tianjin Medical University, 23 Pingjiang Road, 300211, Hexi District, Tianjin, China aut Cytotechnology Springer Netherlands 63/5(2011-10-01), 531-539 0920-9069 63:5<531 2011 63 10616 https://doi.org/10.1007/s10616-011-9376-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9376-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ning Jiwei Research Laboratory for Tumor Immunity, Tianjin Urological Surgery Institute, Second Affiliated Hospital, Tianjin Medical University, 23 Pingjiang Road, 300211, Hexi District, Tianjin, China aut NATIONALLICENCE 700 1- Li Changying Research Laboratory for Tumor Immunity, Tianjin Urological Surgery Institute, Second Affiliated Hospital, Tianjin Medical University, 23 Pingjiang Road, 300211, Hexi District, Tianjin, China aut NATIONALLICENCE 700 1- Li Hongjie Department of Pathophysiology, Institute of Basic Medicine, HeBei United University, 57 Jianshe Road, 063000, Lubei District, Tangshan, China aut NATIONALLICENCE 700 1- Chang Jiwu Research Laboratory for Tumor Immunity, Tianjin Urological Surgery Institute, Second Affiliated Hospital, Tianjin Medical University, 23 Pingjiang Road, 300211, Hexi District, Tianjin, China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 531-539 0920-9069 63:5<531 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer