caa a22 4500 44582333X CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9360-y doi (NATIONALLICENCE)springer-10.1007/s10616-011-9360-y Apoptotic effects of hydrogen peroxide and vitamin C on chicken embryonic fibroblasts: redox state and programmed cell death [Elektronische Daten] [D. Jin, C. Li, H. Yang, W. Zhang, C. Li, W. Guan, Y. Ma] The pro-apoptotic effects of hydrogen peroxide and the purported anti-apoptotic effects of Vitamin C on chicken embryonic fibroblasts were investigated. Hydrogen peroxide induced morphological changes in a dose dependent manner, and a myriad of autophagosomes were observed using transmission electron microscopy. Doxorubicin elicited alterations were not inhibited by co-incubation with Vitamin C except that mitochondrial structure was slightly improved. TUNEL assay, cytotoxicity analysis and flow cytometry revealed that the cytotoxicity, DNA fragmentation and apoptotic rates were dose dependent upon treatment with hydrogen peroxide. Calcium homeostasis was disrupted in a dose dependent manner, and cell cycle was blocked at G2/M checkpoint at low concentration and S/G2 checkpoint at high concentration respectively upon treatment with hydrogen peroxide. The administration of Vitamin C only has a modest effect against doxorubicin induced apoptosis, calcium homeostasis disruption and cell cycle arrest. This research demonstrated that the elevation of reactive oxygen species is sufficient to induce the apoptosis of chicken embryonic fibroblasts, whereas the administration of Vitamin C does not necessarily have certain anti-apoptotic effects, especially when the stimulus is not directly linked with redox state. Springer Science+Business Media B.V., 2011 Chicken nationallicence Embryonic fibroblasts nationallicence Hydrogen peroxide nationallicence Vitamin C nationallicence Apoptosis nationallicence Redox state nationallicence AO : Acridine orange nationallicence CEFs : Chicken embryonic fibroblasts nationallicence EB : Ethidium bromide nationallicence MTT : 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrasolium bromide nationallicence PI : Propidium iodide nationallicence ROS : Reactive oxygen species nationallicence TEM : Transmission electron microscopy nationallicence Jin D. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut Li C. Northeast Forestry University (NFU), 150040, Harbin, Heilongjiang, People's Republic of China aut Yang H. College of Biological Sciences, China Agricultural University (CAU), 100193, Beijing, People's Republic of China aut Zhang W. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut Li C. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut Guan W. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut Ma Y. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut Cytotechnology Springer Netherlands 63/5(2011-10-01), 461-471 0920-9069 63:5<461 2011 63 10616 https://doi.org/10.1007/s10616-011-9360-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9360-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin D. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li C. Northeast Forestry University (NFU), 150040, Harbin, Heilongjiang, People's Republic of China aut NATIONALLICENCE 700 1- Yang H. College of Biological Sciences, China Agricultural University (CAU), 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhang W. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li C. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Guan W. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Ma Y. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), 100193, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 461-471 0920-9069 63:5<461 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer