caa a22 4500 445823348 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9372-7 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9372-7 The effects of 4-thujanol on chromosome aberrations, sister chromatid exchanges and micronucleus in human peripheral blood lymphocytes [Elektronische Daten] [Ayşe Kocaman, Eyyüp Rencüzoğulları, Mehmet Topaktaş, Erman İstifli, Mehmet Büyükleyla] 4-Thujanol, a bicyclic monoterpene alcohol, is present in the essential oils of many medicinal and aromatic plants. It is commonly used as a fragrance and flavouring ingredient in a lot of different products. The potential genotoxic effects of 4-thujanol on human peripheral blood lymphocytes (PBLs) were investigated in vitro by the chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests. The cells were treated with 13, 26 and 52μg/mL 4-thujanol in the presence and absence of a metabolic activator (S9 mix). 4-Thujanol induced CA (P<0.001) and MN formation (P<0.05) at all concentrations (13, 26 and 52μg/mL) in the presence and absence of the S9 mix without a concentration-dependent manner. However, the treatment of peripheral lymphocytes with 4-thujanol did not produce a statistical difference in the frequency of SCEs when compared with control group. Furthermore, this monoterpene did not significantly decrease the mitotic index (MI), proliferation index (PI), and nuclear division index (NDI). In conclusion, 4-thujanol had a significant clastogenic effect at the tested concentrations (13, 26 and 52μg/mL) for human PBLs. In addition, no cytotoxic and/or cytostatic effects were observed regardless of the concentrations used. This work presents the first report on genotoxic properties of 4-thujanol. Springer Science+Business Media B.V., 2011 4-Thujanol nationallicence Human peripheral blood lymphocytes nationallicence Genotoxicity nationallicence Chromosome aberrations nationallicence Sister chromatid exchanges nationallicence Micronucleus nationallicence CA : Chromosome aberration nationallicence SCE : Sister chromatid exchange nationallicence MN : Micronucleus nationallicence PBLs : Peripheral blood lymphocytes nationallicence MNBN : Micronucleated binuclear cell nationallicence MI : Mitotic index nationallicence PI : Proliferation index nationallicence NDI : Nuclear division index nationallicence MMC : Mitomycin-C nationallicence CP : Cyclophosphamide nationallicence Cyt-B : Cytochalasin B nationallicence Kocaman Ayşe Department of Biology, Faculty of Science and Letters, Mustafa Kemal University, 31000, Hatay, Turkey aut Rencüzoğulları Eyyüp Department of Biology, Faculty of Science and Letters, Adıyaman University, Adıyaman, Turkey aut Topaktaş Mehmet Department of Biology, Faculty of Science and Letters, Çukurova University, Adana, Turkey aut İstifli Erman Department of Biology, Basic and Applied Sciences Institute, Çukurova University, Adana, Turkey aut Büyükleyla Mehmet Department of Biology, Basic and Applied Sciences Institute, Çukurova University, Adana, Turkey aut Cytotechnology Springer Netherlands 63/5(2011-10-01), 493-502 0920-9069 63:5<493 2011 63 10616 https://doi.org/10.1007/s10616-011-9372-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9372-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kocaman Ayşe Department of Biology, Faculty of Science and Letters, Mustafa Kemal University, 31000, Hatay, Turkey aut NATIONALLICENCE 700 1- Rencüzoğulları Eyyüp Department of Biology, Faculty of Science and Letters, Adıyaman University, Adıyaman, Turkey aut NATIONALLICENCE 700 1- Topaktaş Mehmet Department of Biology, Faculty of Science and Letters, Çukurova University, Adana, Turkey aut NATIONALLICENCE 700 1- İstifli Erman Department of Biology, Basic and Applied Sciences Institute, Çukurova University, Adana, Turkey aut NATIONALLICENCE 700 1- Büyükleyla Mehmet Department of Biology, Basic and Applied Sciences Institute, Çukurova University, Adana, Turkey aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 493-502 0920-9069 63:5<493 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer