caa a22 4500 445823356 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9365-6 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9365-6 Development of cell line from the testicular tissues of crab Scylla serrata [Elektronische Daten] [Anumol Shashikumar, P. Desai] This is the first report on development of a finite cell line from testicular tissues of crab, Scylla serrata. Both the explant and segregated tissues of testes yielded cells that could proliferate and grow. These cells ranged in size from 10 to 38μm with distinct nuclei of varying shapes. The testicular cells survived and proliferated best in L-15-crab saline medium supplemented with epidermal growth factor (20ng/mL) and glucose (1mg/mL). The cell proliferation rate was assessed by Methyl tetrazolium assay in terms of change in optical density which clearly indicated a prominent increase in cell density. The testicular cells were subcultured at an interval of 4-6days. These subcultured cells remained healthy and proliferated for 5months with a minimum of ten subsequent passages. The finite cell line was characterized in terms of morphology, growth rate, lactate dehydrogenase release (for detecting health status) and 18S rRNA sequencing. This cell line could be a very useful tool for testing infections and replications of crustacean viruses. The present work provides a technique that could be extended for developing other crustacean cell lines. Springer Science+Business Media B.V., 2011 Cell culture nationallicence Cell line nationallicence Scylla serrata nationallicence Crab nationallicence 18S rRNA sequence nationallicence MTT nationallicence EGF : Epidermal growth factor nationallicence FBS : Fetal bovine serum nationallicence HS : Horse serum nationallicence MTT : Methyl tetrazolium nationallicence LDH : Lactate dehydrogenase nationallicence PCN : Product code number nationallicence Shashikumar Anumol Department of Zoology, Goa University, 403206, Taleigao Plateau, Goa, India aut Desai P. Department of Zoology, Goa University, 403206, Taleigao Plateau, Goa, India aut Cytotechnology Springer Netherlands 63/5(2011-10-01), 473-480 0920-9069 63:5<473 2011 63 10616 https://doi.org/10.1007/s10616-011-9365-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9365-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shashikumar Anumol Department of Zoology, Goa University, 403206, Taleigao Plateau, Goa, India aut NATIONALLICENCE 700 1- Desai P. Department of Zoology, Goa University, 403206, Taleigao Plateau, Goa, India aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 473-480 0920-9069 63:5<473 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer