caa a22 4500 445823372 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9375-4 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9375-4 Ramadan Gamal Biological Science Department, College of Science, King Faisal University, Al-Hufof, KSA aut Generation of functional monocyte-derived fast dendritic cells suitable for clinical application in the absence of interleukin-6 [Elektronische Daten] [Gamal Ramadan] To develop dendritic cells (DCs)-based immunotherapy for cancer patients, it is necessary to have a standardized, reproducible, fast, and easy to use protocol for in vitro generation of fully functional DCs. Recently, a new strategy was described for differentiation and maturation of human monocyte (Mo)-derived fast-DCs with full T cell stimulatory capacity within only 48-72h of in vitro culture. Interleukin (IL)-6 plus tumour necrosis factor (TNF)-α, IL-1β, and prostaglandin (PG)-E2 were used in this strategy to induce maturation of the generated DCs. The present study further modifies this strategy by excluding IL-6 from the cytokines cocktail used for DCs maturation. The results showed that maturation of fast-DCs without IL-6 did not significantly alter the morphology, phenotype and the yield of mature DCs (P>0.05, compared with those generated with IL-6). Moreover, fast-DCs generated without IL-6 are functional antigen presenting cells, have the ability to induce tetanus toxoid-specific autologous T cell proliferation, and are suitable for gene delivery through adenoviral vector transduction as those generated with IL-6 (P>0.05). In conclusion, the present study proves that fully mature and functional Mo-derived fast-DCs can be generated in vitro without adding IL-6, which not only reduces the number of required recombinant cytokines, but may also resemble DCs development in vivo more closely. Springer Science+Business Media B.V., 2011 Adenoviral expression nationallicence Interleukin-6 nationallicence Mo-derived fast-DCs nationallicence T cell proliferation nationallicence Tetanus toxoid nationallicence Cytotechnology Springer Netherlands 63/5(2011-10-01), 513-521 0920-9069 63:5<513 2011 63 10616 https://doi.org/10.1007/s10616-011-9375-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9375-4 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Ramadan Gamal Biological Science Department, College of Science, King Faisal University, Al-Hufof, KSA aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 513-521 0920-9069 63:5<513 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer