caa a22 4500 445823380 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9371-8 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9371-8 Immunostimulatory effects of collagen from jellyfish in vivo [Elektronische Daten] [Hitoshi Morishige, Takuya Sugahara, Sogo Nishimoto, Ayako Muranaka, Fumi Ohno, Ryusuke Shiraishi, Mikiharu Doi] We focused on the biological activity of the collagen extracts obtained from the giant edible jellyfish, Nemopilema nomurai. Jellyfish collagen extracts stimulates the production of immunoglobulins (Igs) and cytokines by human hybridoma cells and human peripheral blood lymphocytes. Therefore, we examined the immunoregulatory function of jellyfish collagen extracts in mice. Intake of jellyfish collagen extracts facilitated the Ig production activity of lymphocytes from spleen and Peyer's patch. Furthermore, the levels of Igs in the serum clearly increased after the administration of jellyfish collagen extracts. Intake of bovine collagen from Achilles' tendon also activated lymphocytes activity in mice. The activity of total and antigen-specific Ig production in splenocytes from OVA-challenged mice was also enhanced by collagen intake. However, the total and OVA-specific IgE levels in the serum were not affected by the collagen intake. These results suggested that jellyfish collagen extracts stimulates an immune response in vivo, without inducing allergic complications. Springer Science+Business Media B.V., 2011 Collagen nationallicence Immunostimulation nationallicence Immunoglobulin production nationallicence Jellyfish nationallicence Lymphocyte nationallicence BSA : Bovine serum albumin nationallicence ELISA : Enzyme-linked immunosorbent assay nationallicence FBS : Fetal bovine serum nationallicence IFN-γ : Interferon-γ nationallicence Ig : Immunoglobulin nationallicence IL-4 : Interleukin 4 nationallicence IPSF : Immunoglobulin production stimulating factor nationallicence MLN : Mesenteric lymph node nationallicence NaPB : Sodium phosphate buffer nationallicence OVA : Ovalbumin nationallicence PBL : Peripheral blood lymphocyte nationallicence PBS : Phosphate buffered saline nationallicence PP : Peyer's patch nationallicence TNF-α : Tumor necrosis factor-α nationallicence Morishige Hitoshi Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut Sugahara Takuya Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut Nishimoto Sogo Center for Marine Environmental Studies, Ehime University, 2-5 Bunkyo-cho, 790-8577, Matsuyama, Ehime, Japan aut Muranaka Ayako Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut Ohno Fumi Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut Shiraishi Ryusuke Marutomo Co., Ltd., 1696 Kominato, 799-3192, Iyo, Ehime, Japan aut Doi Mikiharu Marutomo Co., Ltd., 1696 Kominato, 799-3192, Iyo, Ehime, Japan aut Cytotechnology Springer Netherlands 63/5(2011-10-01), 481-492 0920-9069 63:5<481 2011 63 10616 https://doi.org/10.1007/s10616-011-9371-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9371-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morishige Hitoshi Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Sugahara Takuya Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Nishimoto Sogo Center for Marine Environmental Studies, Ehime University, 2-5 Bunkyo-cho, 790-8577, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Muranaka Ayako Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Ohno Fumi Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, 790-8566, Matsuyama, Ehime, Japan aut NATIONALLICENCE 700 1- Shiraishi Ryusuke Marutomo Co., Ltd., 1696 Kominato, 799-3192, Iyo, Ehime, Japan aut NATIONALLICENCE 700 1- Doi Mikiharu Marutomo Co., Ltd., 1696 Kominato, 799-3192, Iyo, Ehime, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 481-492 0920-9069 63:5<481 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer