caa a22 4500 445823402 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10616-011-9373-6 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9373-6 Comparative cellular and molecular analysis of cytotoxicity and apoptosis induction by doxorubicin and Baneh in human breast cancer T47D cells [Elektronische Daten] [P. Rezaei, Sh. Fouladdel, Silvia Cristofanon, S. Ghaffari, G. Amin, E. Azizi] It is now widely accepted that dietary phytochemicals inhibit cancer progression and enhance the effects of conventional chemotherapy. In this report, we comparatively studied the cellular and molecular aspects of apoptosis induction by the methanolic extract of Baneh fruit skin in comparison to Doxorubicin (Dox), a well-known anticancer drug, in human breast cancer T47D cells. The MTT assay was used to determine the antiproliferative effects. The flow cytometric and microscopic analyses were done to evaluate the apoptosis induction. Furthermore, western blot analyses have been done to study the role of key molecular players of apoptosis including caspase 3 and PARP. The Baneh extract showed strong antiproliferative activity against T47D cells in a dose- and time-dependent manner that was comparable to and even stronger than Dox in certain concentrations. Analysis of Baneh-treated cells by flow cytometry and fluorescence microscopy indicated strong apoptosis induction and nuclear morphological alterations similar to or greater than Dox. Finally, molecular analysis of apoptosis by western blotting proved activation of caspase 3 followed by poly ADP ribose polymerase (PARP) cleavage more efficiently in Baneh than in Dox treated cancer cells. These findings indicate that Baneh extract contains phytochemicals which act as inhibitor of cell proliferation and inducer of apoptosis in human breast cancer T47D cells that makes it a potentially good candidate for new anticancer drug development. Springer Science+Business Media B.V., 2011 Caspase3 nationallicence PARP nationallicence Apoptosis nationallicence Breast cancer nationallicence Baneh nationallicence Doxorubicin nationallicence AnnV : Annexin-V-FITC nationallicence Baneh : Pistacia atlantica sub kurdica nationallicence DAPI : 4′,6-diamidine-2′-phenylindole dihydrochloride nationallicence Dox : Doxorubicin nationallicence IC50 : Half maximal inhibitory concentration nationallicence MTT : 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide nationallicence NF- κ B : Nuclear factor κ B nationallicence PARP : Poly ADP ribose polymerase nationallicence PI : Propidium Iodide nationallicence Rezaei P. Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Fouladdel Sh Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Cristofanon Silvia University Children's Hospital, Ulm University, Ulm, Germany aut Ghaffari S. Department of Biochemistry, IBB, University of Tehran, Tehran, Iran aut Amin G. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Azizi E. Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut Cytotechnology Springer Netherlands 63/5(2011-10-01), 503-512 0920-9069 63:5<503 2011 63 10616 https://doi.org/10.1007/s10616-011-9373-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9373-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rezaei P. Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 700 1- Fouladdel Sh Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 700 1- Cristofanon Silvia University Children's Hospital, Ulm University, Ulm, Germany aut NATIONALLICENCE 700 1- Ghaffari S. Department of Biochemistry, IBB, University of Tehran, Tehran, Iran aut NATIONALLICENCE 700 1- Amin G. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 700 1- Azizi E. Molecular Research Lab, Department of Pharmacology and Toxicology, Tehran University of Medical Sciences (TUMS), Tehran, Iran aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/5(2011-10-01), 503-512 0920-9069 63:5<503 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer