caa a22 4500 445823410 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-010-9323-8 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9323-8 Inhibitory effect of hot-water extract of quince ( Cydonia oblonga ) on immunoglobulin E-dependent late-phase immune reactions of mast cells [Elektronische Daten] [Takeshi Kawahara, Tatsuhiro Iizuka] We evaluated the effect of a crude hot-water extract (HW) of quince (Cydonia oblonga Miller) fruit on immunoglobulin E (IgE)-dependent late-phase immune reactions of mast cells using in vitro system. Mast cell-like RBL-2H3 cells were treated with quince HW and late-phase reaction was then induced by stimulation with IgE+Antigen. Quince HW reduced the elevation of interleukin-13 and tumor necrosis factor-α expression level. Furthermore, quince HW suppressed these cytokine expressions of mouse bone marrow-derived mast cells (BMMCs), a normal mast cell model. Leukotriene C4 and prostaglandin D2 production in BMMCs after 1 and 6h of stimulation, respectively, were also reduced by treating the cells with quince HW. We found that the induction of intracellular cyclooxygenase (COX)-2 expression but not COX-1 expression in BMMCs was reduced by quince HW. These results suggest that quince HW has an inhibitory effect on broad range of the late-phase immune reactions of mast cells. Springer Science+Business Media B.V., 2011 Quince nationallicence Mast cell nationallicence Late-phase reaction nationallicence Leukotriene C4 nationallicence Prostaglandin D2 nationallicence Cyclooxygenase nationallicence Ag : Antigen nationallicence BMMC : Bone marrow-derived mast cell nationallicence COX : Cyclooxygenase nationallicence ELISA : Enzyme-linked immunosorbent assay nationallicence FBS : Fetal bovine serum nationallicence FcεRI : High-affinity IgE receptor nationallicence FITC : Fluorescein isothiocyanate nationallicence GAPDH : Glyceraldehyde-3-phosphate dehydrogenase nationallicence HW : Hot-water extract nationallicence IgE : Immunoglobulin E nationallicence IL : Interleukin nationallicence LT : Leukotriene nationallicence NSAID : Nonsteroidal anti-inflammatory drug nationallicence PBS : Phosphate-buffered saline nationallicence PE : Phycoerythrin nationallicence PG : Prostaglandin nationallicence RT-PCR : Reverse transcription-polymerase chain reaction nationallicence SD : Standard deviation nationallicence TNF : Tumor necrosis factor nationallicence Kawahara Takeshi Laboratory of Food Bioscience, Faculty of Agriculture, Shinshu University, 8304, 399-4598, Minamiminowa, Kamiina, Nagano, Japan aut Iizuka Tatsuhiro Laboratory of Food Bioscience, Faculty of Agriculture, Shinshu University, 8304, 399-4598, Minamiminowa, Kamiina, Nagano, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 143-152 0920-9069 63:2<143 2011 63 10616 https://doi.org/10.1007/s10616-010-9323-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9323-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawahara Takeshi Laboratory of Food Bioscience, Faculty of Agriculture, Shinshu University, 8304, 399-4598, Minamiminowa, Kamiina, Nagano, Japan aut NATIONALLICENCE 700 1- Iizuka Tatsuhiro Laboratory of Food Bioscience, Faculty of Agriculture, Shinshu University, 8304, 399-4598, Minamiminowa, Kamiina, Nagano, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 143-152 0920-9069 63:2<143 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer