caa a22 4500 445823437 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-010-9308-7 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9308-7 Screening of substrate peptide sequences for tissue-type transglutaminase (TGase 2) using T7 phage cDNA library [Elektronische Daten] [Yoshiaki Sugimura, Hiroyuki Yamashita, Kiyotaka Hitomi] Transglutaminase (TGase) is a family of enzymes that catalyzes cross-linking reaction between glutamine- and lysine residue of substrate proteins in several mammalian biological events. Substrate proteins for TGase and their physiological relevance have been still in research, continuously expanding. In this study, we have established a novel screening system that enables identification of cDNA sequence encoding favorable primary structure as a substrate for tissue-type transglutaminase (TGase 2), a multifunctional and ubiquitously expressing isozyme. By the screening, we identified several T7 phage clones that displayed substrate peptides for TGase 2 as a translated product from human brain cDNA library. Among the selected clones, the C-terminal region of IKAP, IkappaB kinase complex associated protein, appeared as a highly reactive substrate sequence for TGase 2. This system will open possibility of rapid identification of substrate sequences for transglutaminases at a genetic level. Springer Science+Business Media B.V., 2010 Transglutaminase nationallicence cDNA library nationallicence T7 phage nationallicence IKAP nationallicence Calcium nationallicence Sugimura Yoshiaki Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Yamashita Hiroyuki Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Hitomi Kiyotaka Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 111-118 0920-9069 63:2<111 2011 63 10616 https://doi.org/10.1007/s10616-010-9308-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9308-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugimura Yoshiaki Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Yamashita Hiroyuki Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 700 1- Hitomi Kiyotaka Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, 464-8601, Nagoya, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 111-118 0920-9069 63:2<111 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer