caa a22 4500 445823445 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-011-9341-1 doi (NATIONALLICENCE)springer-10.1007/s10616-011-9341-1 3,4,5-tri- O -caffeoylquinic acid inhibits amyloid β-mediated cellular toxicity on SH-SY5Y cells through the upregulation of PGAM1 and G3PDH [Elektronische Daten] [Yusaku Miyamae, Junkyu Han, Kazunori Sasaki, Mika Terakawa, Hiroko Isoda, Hideyuki Shigemori] Caffeoylquinic acid (CQA) is one of the phenylpropanoids found in a variety of natural resources and foods, such as sweet potatoes, propolis, and coffee. Previously, we reported that 3,5-di-O-caffeoylquinic acid (3,5-di-CQA) has a neuroprotective effect against amyloid-β (Aβ)-induced cell death through the overexpression of glycolytic enzyme. Additionally, 3,5-di-CQA administration induced the improvement of spatial learning and memory on senescence accelerated-prone mice (SAMP8). The aim of this study was to investigate whether 3,4,5-tri-O-caffeoylquinic acid (3,4,5-tri-CQA), isolated from propolis, shows a neuroprotective effect against Aβ-induced cell death on human neuroblastoma SH-SY5Y cells. To clarify the possible mechanism, we performed proteomics and real-time RT-PCR as well as a measurement of the intracellular adenosine triphosphate (ATP) level. These results showed that 3,4,5-tri-CQA attenuated the cytotoxicity and prevented Aβ-mediated apoptosis. Glycolytic enzymes, phosphoglycerate mutase 1 (PGAM1) and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) were overexpressed in co-treated cells with both 3,4,5-tri-CQA and Aβ. The mRNA expression of PGAM1, G3PDH, and phosphoglycerate kinase 1 (PGK1), and intracellular ATP level were also increased in 3,4,5-tri-CQA treated cells. Taken together the findings in our study suggests that 3,4,5-tri-CQA shows a neuroprotective effect against Aβ-induced cell death through the upregulation of glycolytic enzyme mRNA as well as ATP production activation. Springer Science+Business Media B.V., 2011 3,4,5-tri- O -caffeoylquinic acid nationallicence SH-SY5Y cells nationallicence G3PDH nationallicence PGAM1 nationallicence Glycolytic enzyme nationallicence ATP nationallicence Miyamae Yusaku Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut Han Junkyu Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut Sasaki Kazunori Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut Terakawa Mika Kato Brothers Honey Co., Ltd., 236-0004, Yokohama, Kanagawa, Japan aut Isoda Hiroko Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut Shigemori Hideyuki Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 191-200 0920-9069 63:2<191 2011 63 10616 https://doi.org/10.1007/s10616-011-9341-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-011-9341-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyamae Yusaku Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Han Junkyu Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sasaki Kazunori Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Terakawa Mika Kato Brothers Honey Co., Ltd., 236-0004, Yokohama, Kanagawa, Japan aut NATIONALLICENCE 700 1- Isoda Hiroko Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Shigemori Hideyuki Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 191-200 0920-9069 63:2<191 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer