caa a22 4500 445823453 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-010-9317-6 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9317-6 Suppressive effects of electrolyzed reduced water on alloxan-induced apoptosis and type 1 diabetes mellitus [Elektronische Daten] [Yupin Li, Takeki Hamasaki, Noboru Nakamichi, Taichi Kashiwagi, Takaaki Komatsu, Jun Ye, Kiichiro Teruya, Masumi Abe, Hanxu Yan, Tomoya Kinjo, Shigeru Kabayama, Munenori Kawamura, Sanetaka Shirahata] Electrolyzed reduced water, which is capable of scavenging reactive oxygen species, is attracting recent attention because it has shown improved efficacy against several types of diseases including diabetes mellitus. Alloxan produces reactive oxygen species and causes type 1 diabetes mellitus in experimental animals by irreversible oxidative damage to insulin-producing β-cells. Here, we showed that electrolyzed reduced water prevented alloxan-induced DNA fragmentation and the production of cells in sub-G1 phase in HIT-T15 pancreatic β-cells. Blood glucose levels in alloxan-induced type 1 diabetes model mice were also significantly suppressed by feeding the mice with electrolyzed reduced water. These results suggest that electrolyzed reduced water can prevent apoptosis of pancreatic β-cells and the development of symptoms in type 1 diabetes model mice by alleviating the alloxan-derived generation of reactive oxygen species. Springer Science+Business Media B.V., 2010 Electrolyzed reduced water nationallicence Alloxan nationallicence Type 1 diabetes mellitus nationallicence Reactive oxygen species nationallicence HIT-T15 cells nationallicence ALX : Alloxan nationallicence BSA : Bovine serum albumin nationallicence EDTA : Ethylenediaminetetraacetic acid nationallicence ERW : Electrolyzed reduced water nationallicence FBS : Fetal bovine serum nationallicence DM : Diabetes mellitus nationallicence HBSS : Hank's balanced salt solution nationallicence HEPES : 4-[2-hydroxyethyl]-1-piperazineethane-sulfonic acid nationallicence PBS : Phosphate buffered saline nationallicence PI : Propidium iodide nationallicence ROS : Reactive oxygen species nationallicence T1DM : Type 1 diabetes mellitus nationallicence T2DM : Type 2 diabetes mellitus nationallicence TdT : Terminal deoxynucleotidyl transferase nationallicence Li Yupin Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Hamasaki Takeki Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Nakamichi Noboru Nihon Trim Co Ltd, 1-8-34 Oyodonaka, Kita-ku, 531-0076, Osaka, Japan aut Kashiwagi Taichi Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Komatsu Takaaki Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Ye Jun Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Teruya Kiichiro Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Abe Masumi Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Yan Hanxu Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Kinjo Tomoya Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Kabayama Shigeru Nihon Trim Co Ltd, 1-8-34 Oyodonaka, Kita-ku, 531-0076, Osaka, Japan aut Kawamura Munenori Kyowa Hospital, 191-1 Sakae, Osibetanimachi, Nishi-ku, 851-2211, Kobe, Japan aut Shirahata Sanetaka Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 119-131 0920-9069 63:2<119 2011 63 10616 https://doi.org/10.1007/s10616-010-9317-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9317-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yupin Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Hamasaki Takeki Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nakamichi Noboru Nihon Trim Co Ltd, 1-8-34 Oyodonaka, Kita-ku, 531-0076, Osaka, Japan aut NATIONALLICENCE 700 1- Kashiwagi Taichi Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Komatsu Takaaki Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ye Jun Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Teruya Kiichiro Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Abe Masumi Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Yan Hanxu Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kinjo Tomoya Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kabayama Shigeru Nihon Trim Co Ltd, 1-8-34 Oyodonaka, Kita-ku, 531-0076, Osaka, Japan aut NATIONALLICENCE 700 1- Kawamura Munenori Kyowa Hospital, 191-1 Sakae, Osibetanimachi, Nishi-ku, 851-2211, Kobe, Japan aut NATIONALLICENCE 700 1- Shirahata Sanetaka Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 119-131 0920-9069 63:2<119 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer