caa a22 4500 44582347X CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-010-9321-x doi (NATIONALLICENCE)springer-10.1007/s10616-010-9321-x Interleukin-12 inhibits development of ectopic endometriotic tissues in peritoneal cavity via activation of NK cells in a murine endometriosis model [Elektronische Daten] [Hiroyuki Itoh, Toshihiro Sashihara, Akira Hosono, Shuichi Kaminogawa, Masayuki Uchida] Involvement of impaired peritoneal immunosurveillance systems has been well established in the pathology of endometriosis. On the other hand, it has been observed that peritoneal administration of IL-12 suppress development of endometriotic lesions in a mouse endometriosis model. We investigated the effect of peritoneal administration of IL-12 on the peritoneal immunosurveillance system regarding NK cells in the mouse model. Treating the endometrial-tissue challenged mice with IL-12 for 5 consecutive days, from day -2 to day 2 (implantation of the endometrial tissues was done on day 0), cytotoxicity of splenic NK cells was enhanced immediately after the administration, on day 3, and development of the endometriotic lesions was reduced on day 21. In vivo NK cell depletion by administration of anti-IL-2Rβ mAb resulted in reduction of the cytotoxicity of splenic NK cells concomitant with a significant attenuation of suppressive effect of IL-12 on development of endometriotic lesions. Therefore, it was suggested that IL-12 suppresses development of endometriotic lesions via activation of NK cells, and that NK cells are involved in the primary defense for the development of endometriotic lesions. Springer Science+Business Media B.V., 2011 Endometriosis nationallicence Interleukin-12 nationallicence Natural killer cells nationallicence Murine model nationallicence IL : Interleukin nationallicence INF : Interferon nationallicence NK : Natural killer nationallicence Itoh Hiroyuki Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540 Naruda, 250-0862, Odawara, Kanagawa, Japan aut Sashihara Toshihiro Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540 Naruda, 250-0862, Odawara, Kanagawa, Japan aut Hosono Akira Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-8510, Fujisawa, Kanagawa, Japan aut Kaminogawa Shuichi Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-8510, Fujisawa, Kanagawa, Japan aut Uchida Masayuki Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540 Naruda, 250-0862, Odawara, Kanagawa, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 133-141 0920-9069 63:2<133 2011 63 10616 https://doi.org/10.1007/s10616-010-9321-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9321-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Itoh Hiroyuki Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540 Naruda, 250-0862, Odawara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sashihara Toshihiro Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540 Naruda, 250-0862, Odawara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Hosono Akira Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-8510, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Kaminogawa Shuichi Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-8510, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Uchida Masayuki Food Science Institute, Division of Research and Development, Meiji Dairies Corporation, 540 Naruda, 250-0862, Odawara, Kanagawa, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 133-141 0920-9069 63:2<133 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer