caa a22 4500 445823488 CHVBK 20180317145251.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-010-9333-6 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9333-6 Microarray analysis of immediate-type allergy in KU812 cells in response to fulvic acid [Elektronische Daten] [Hideko Motojima, Myra O. Villareal, Junkyu Han, Hiroko Isoda] Fulvic acid (FA) is class of compounds of humic substances formed through the degradation of organic substances by chemical and biological processes. FA has been utilized in traditional Chinese medicine and possesses various pharmacological properties. Previously, we reported that FA extracted from solubilized excess sludge (SS-FA) had an inhibitory effect on β-hexosaminidase release in human leukemia basophilic (KU812) cells. In this study, we investigated the effects of SS-FA on the immediate-type allergic reaction and studied its possible mechanisms of action in KU812 cells following activation with phorbol myristate acetate (20nmol L−1) plus calcium ionophore A23187 (1μmol L−1) (PMACI). The inhibitory effect of SS-FA on degranulation in PMACI-stimulated KU812 cells was examined using histamine release assay. SS-FA significantly decreased the histamine release in KU812 cells at concentrations of 0.1-10.0μgmL−1. To gain more information regarding the mechanism of the suppression of degranulation following SS-FA treatment, microarray was conducted to determine which genes were differentially expressed in response to SS-FA in PMACI-activated KU812 cells. From a total of 201 genes in the DNA chip, 28 genes were up-regulated and 173 genes were down-regulated in cells pretreated with SS-FA for 15min and stimulated with PMACI. From the 71 genes that showed more than two fold change in expression, 16 genes were significantly down-regulated that were subjected to hierarchical clustering. SS-FA affected the expression of genes that were involved in the following pathways: signal transduction, cytokine-cytokine receptor interaction, immune response, cell adhesion molecules and IgE receptor β subunit response. Springer Science+Business Media B.V., 2011 Immediate-type allergy nationallicence Histamine release nationallicence Microarray analysis nationallicence Fulvic acid nationallicence Solubilized excess sludge nationallicence DNP-BSA : Dinitrophenylated bovine serum albumin nationallicence FA : Fulvic acid nationallicence FBS : Fetal bovine serum nationallicence KU812 : Human leukemia basophilic nationallicence PMA : Phorbol 12-myristate 13-acetate nationallicence PMACI : PMA plus calcium ionophore A23187 nationallicence RBL-2H3 : Rat basophilic leukemia nationallicence SLE : Systemic lupus erythematosus nationallicence SS-FA : Fulvic acid extracted from solubilized sludge nationallicence Motojima Hideko Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut O. Villareal Myra Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut Han Junkyu Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut Isoda Hiroko Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 181-190 0920-9069 63:2<181 2011 63 10616 https://doi.org/10.1007/s10616-010-9333-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9333-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Motojima Hideko Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- O. Villareal Myra Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Han Junkyu Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 700 1- Isoda Hiroko Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Ibaraki, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 181-190 0920-9069 63:2<181 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer