caa a22 4500 445823496 CHVBK 20180317145252.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-010-9330-9 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9330-9 Enhancement of antibody production by the addition of Coenzyme-Q10 [Elektronische Daten] [Yoshinobu Konno, Motoi Aoki, Masakazu Takagishi, Naoto Sakai, Masamichi Koike, Kaori Wakamatsu, Shinji Hosoi] Recently, there has been a growing demand for therapeutic monoclonal antibodies (MAbs) on the global market. Because therapeutic MAbs are more expensive than low-molecular-weight drugs, there have been strong demands to lower their production costs. Therefore, efficient methods to minimize the cost of goods are currently active areas of research. We have screened several enhancers of specific MAb production rate (SPR) using a YB2/0 cell line and found that coenzyme-Q10 (CoQ10) is a promising enhancer candidate. CoQ10 is well known as a strong antioxidant in the respiratory chain and is used for healthcare and other applications. Because CoQ10 is negligibly water soluble, most studies are limited by low concentrations. We added CoQ10 to a culture medium as dispersed nanoparticles at several concentrations (Q-Media) and conducted a fed-batch culture. Although the Q-Media had no effect on cumulative viable cell density, it enhanced SPR by 29%. In addition, the Q-Media had no effect on the binding or cytotoxic activity of MAbs. Q-Media also enhanced SPR with CHO and NS0 cell lines by 30%. These observations suggest that CoQ10 serves as a powerful aid in the production of MAbs by enhancing SPR without changing the characteristics of cell growth, or adversely affecting the quality or biological activity of MAbs. Springer Science+Business Media B.V., 2010 Antioxidant nationallicence Coenzyme-Q10 nationallicence Chinese hamster ovary (CHO) nationallicence 8-hydroxy-2′-deoxyguanosine nationallicence Monoclonal antibodies nationallicence NS0 nationallicence Specific production rate nationallicence YB2/0 nationallicence Enhancer nationallicence Productivity nationallicence MAbs : Monoclonal antibodies nationallicence CoQ10 : Coenzyme-Q10 nationallicence Q-Media : Culture media supplemented with dispersed nanoparticles of Q10 nationallicence 8OHdG : 8-hydroxy-2′-deoxyguanosine nationallicence SPR : Specific MAb production rate (pg cell−1 d−1) nationallicence Konno Yoshinobu Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., Ltd., 100-1 Hagiwara-machi, Takasaki-shi, 370-0013, Gunma, Japan aut Aoki Motoi Kyowa Hakko Kirin Co., Ltd., 1-6-1 Ohte-machi, Chiyoda-ku, Tokyo, Japan aut Takagishi Masakazu Kyowa Hakko Kirin Co., Ltd., Tokyo Branch, 36-2 Nihonbashi Hakozaki-cho, Chuo-ku, Tokyo, Japan aut Sakai Naoto Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., Ltd., 100-1 Hagiwara-machi, Takasaki-shi, 370-0013, Gunma, Japan aut Koike Masamichi BioWa, Inc., Princeton Commerce Center, 20 Emmons Drive, Suite C-10, 08540, Princeton, NJ, USA aut Wakamatsu Kaori Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, 376-8515, Kiryu-shi, Japan aut Hosoi Shinji Kyowa Hakko Kirin Co., Ltd., 1-6-1 Ohte-machi, Chiyoda-ku, Tokyo, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 163-170 0920-9069 63:2<163 2011 63 10616 https://doi.org/10.1007/s10616-010-9330-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9330-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Konno Yoshinobu Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., Ltd., 100-1 Hagiwara-machi, Takasaki-shi, 370-0013, Gunma, Japan aut NATIONALLICENCE 700 1- Aoki Motoi Kyowa Hakko Kirin Co., Ltd., 1-6-1 Ohte-machi, Chiyoda-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Takagishi Masakazu Kyowa Hakko Kirin Co., Ltd., Tokyo Branch, 36-2 Nihonbashi Hakozaki-cho, Chuo-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Sakai Naoto Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., Ltd., 100-1 Hagiwara-machi, Takasaki-shi, 370-0013, Gunma, Japan aut NATIONALLICENCE 700 1- Koike Masamichi BioWa, Inc., Princeton Commerce Center, 20 Emmons Drive, Suite C-10, 08540, Princeton, NJ, USA aut NATIONALLICENCE 700 1- Wakamatsu Kaori Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, 376-8515, Kiryu-shi, Japan aut NATIONALLICENCE 700 1- Hosoi Shinji Kyowa Hakko Kirin Co., Ltd., 1-6-1 Ohte-machi, Chiyoda-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 163-170 0920-9069 63:2<163 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer