caa a22 4500 445823534 CHVBK 20180317145252.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s10616-010-9331-8 doi (NATIONALLICENCE)springer-10.1007/s10616-010-9331-8 Effect of green tea powder ( Camellia sinensis L. cv. Benifuuki) particle size on O -methylated EGCG absorption in rats; The Kakegawa Study [Elektronische Daten] [Mari Maeda-Yamamoto, Kaori Ema, Yoshiko Tokuda, Manami Monobe, Hirofumi Tachibana, Yoichi Sameshima, Shinichi Kuriyama] Tea polyphenols, e.g., (-)-epigallocatechin-3-O-(3-O-methyl gallate (EGCG3”Me), (-)-epigallocatechin-3-O-gallate (EGCG), (-)-epigallocatechin (EGC), (-)-epicatechin-3-O-gallate (ECG), and (-)-epicatechin (EC), are believed to be responsible for the beneficial effects of tea. ‘Benifuuki', a tea (Camellia sinensis L.) cultivar grown in Japan, is rich in the anti-allergic molecule epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3”Me). Pulverized Benifuuki green tea powder (BGP) is more widely distributed than leaf tea in Japan. Japanese people mix their pulverized tea with water directly, whereas it is common to drink leaf tea after extraction. However, few studies of the effects of BGP particle size on polyphenol bioavailability have been performed. This study was conducted to investigate the absorption of catechins in rats after the intragastric administration of Benifuuki green tea. Therefore, we assessed the plasma concentrations of catechins following the ingestion of BGP with different mean particle sizes (2.86, 18.6, and 76.1μm) or Benifuuki green tea infusion (BGI) as a control in rats. The bioavailabilities of EGCG3”Me, EGCG, ECG, EGC, and EC were analyzed after the oral administration of a single dose of Benifuuki green tea (125mg/rat) to rats. The plasma concentrations of tea catechins were determined by HPLC analysis combined with of electrochemical detection (ECD) using a coulometric array. The AUC (area under the drug concentration versus time curve; min μg/mL) of ester-type catechins (EGCG3”Me, EGCG, and ECG) for the BGP 2.86μm were significantly higher than those in the infusion and 18.6 and 76.1μm BGP groups, but the AUC of free-type catechins (EGC and EC) showed no differences between these groups. Regarding the peak plasma level of EGCG3”Me adjusted for intake, BGP 2.86μm and BGI showed higher values than the BGP 18.6 and 76.1μm groups, and the peak plasma levels of the other catechins displayed the same tendency. The present study demonstrates that the bioavailability of ester-type catechins (EGCG and ECG) can be improved by reducing the particle size of green tea, but the plasma level of EGCG3”Me in the BGI group was similar to that in the BGP 2.86μm group. This result suggests that drinking Benifuuki green tea with a particle size of around 2μm would deliver the anti-allergic EGCG3”Me and the anti-oxidant EGCG efficiently. Springer Science+Business Media B.V., 2011 O -methylated EGCG nationallicence Absorption nationallicence ‘Benifuuki' green tea powder particle size nationallicence Anti-allergic effect nationallicence EGCG : (-)-Epigallocatechin-3-O-gallate nationallicence EGCG3”Me : (-)-Epigallocatechin-3-O-(3-O-methyl) gallate nationallicence ECG : (-)-Epicatechin-3-O-gallate nationallicence EGC : (-)-Epigallocatechin nationallicence EC : (-)-Epicatechin nationallicence BGI : Benifuuki green tea infusion nationallicence BGP : Benifuuki green tea powder nationallicence HPLC : High-performance liquid chromatography nationallicence AUC : Area under the plasma concentration versus time curve nationallicence Cmax : The maximum plasma concentration nationallicence Tmax : Time to reach the maximum plasma concentration nationallicence i.g. : Intragastric(ally) nationallicence Maeda-Yamamoto Mari National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut Ema Kaori National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut Tokuda Yoshiko National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut Monobe Manami National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut Tachibana Hirofumi Bioscience & Biotechnology Faculty of Agriculture, Graduate School of Bioenvironmental Sciences, Kyushu University, 6-10-1 Higashiku, Hakozaki, 812-8581, Fukuoka, Japan aut Sameshima Yoichi Kakegawa City General Hospital, 1-1-1 Sugiya-Minami, 436-8502, Kakegawa, Shizuoka, Japan aut Kuriyama Shinichi Department of Molecular Epidemiology, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, 2-1, Seiryo-Machi, Aoba-Ku, 980-8575, Sendai, Miyagi, Japan aut Cytotechnology Springer Netherlands 63/2(2011-03-01), 171-179 0920-9069 63:2<171 2011 63 10616 https://doi.org/10.1007/s10616-010-9331-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10616-010-9331-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maeda-Yamamoto Mari National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut NATIONALLICENCE 700 1- Ema Kaori National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut NATIONALLICENCE 700 1- Tokuda Yoshiko National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut NATIONALLICENCE 700 1- Monobe Manami National Institute of Vegetable and Tea Sciences, National Agriculture and Food Research Organization, 2769 Kanaya-Shishidoi, 428-8501, Shimada, Shizuoka, Japan aut NATIONALLICENCE 700 1- Tachibana Hirofumi Bioscience & Biotechnology Faculty of Agriculture, Graduate School of Bioenvironmental Sciences, Kyushu University, 6-10-1 Higashiku, Hakozaki, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Sameshima Yoichi Kakegawa City General Hospital, 1-1-1 Sugiya-Minami, 436-8502, Kakegawa, Shizuoka, Japan aut NATIONALLICENCE 700 1- Kuriyama Shinichi Department of Molecular Epidemiology, Environment and Genome Research Center, Tohoku University Graduate School of Medicine, 2-1, Seiryo-Machi, Aoba-Ku, 980-8575, Sendai, Miyagi, Japan aut NATIONALLICENCE 773 0- Cytotechnology Springer Netherlands 63/2(2011-03-01), 171-179 0920-9069 63:2<171 2011 63 10616 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer