caa a22 4500 445824417 CHVBK 20180317145254.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00246-011-0019-4 doi (NATIONALLICENCE)springer-10.1007/s00246-011-0019-4 Establishment of Secondary Iron Overloaded Mouse Model: Evaluation of Cardiac Function and Analysis According to Iron Concentration [Elektronische Daten] [Se Moon, Ji Han, Hui Hwang, Mee Kim, Soon Lee, Jae Lee, Chang Oh, Dae Jeong] Periodic blood transfusion can lead to secondary iron overload in patients with hematologic and oncologic diseases. Iron overload can result in iron deposition in heart tissue, which decreases cardiac function and can ultimately lead to death due to dilated cardiomyopathy and cardiac failure. In this study, we established murine model of secondary iron overload, studied the changes in cardiac function with echocardiography, and examined the histopathologic changes. Three experimental groups of the six week-old C57/BL mice (H-2b) were injected intraperitoneally with 10mg of iron dextran daily 5days a week for 2, 4, and 6weeks. Cumulative doses of iron for the three experimental groups were 100, 200, and 300mg, while the control groups were injected with the same amounts of phosphate-buffered saline. We studied the cardiac function under anesthesia with echocardiography using a GE Vivid7 Dimension system. Plasma iron levels and liver iron contents were measured. The hearts and livers were harvested and stained with H&E and Perls Prussian blue for iron, and the levels of iron deposit were examined. We assessed the cardiac measurements after adjustment for weight. On echocardiography, thicknesses of the interventricular septum and posterior ventricular wall (PS) during diastole showed correlation with the amount of iron deposit (P<0.01). End-diastolic volume showed dilatation of the left ventricle in the 300mg group (P<0.01). Changes in the fractional shortening were not statistically significant (P=0.07). Plasma iron levels and liver iron contents were increased proportionally according to the amount of iron loaded. The histopathologic findings of PS and liver showed higher grade of iron deposit proportional to the cumulated iron dose. In this study, we present an animal model which helps understand the cardiac function changes in patients with secondary iron overload due to repeated blood transfusions. Our results may help characterize the pathophysiologic features of cardiomyopathy in patients with secondary iron overload, and our model may be applied to in vivo iron-chelating therapy studies. Springer Science+Business Media, LLC, 2011 Secondary iron overload nationallicence Cardiac function nationallicence Pathology nationallicence Moon Se Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Han Ji Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Hwang Hui Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Kim Mee Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Lee Soon Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Lee Jae Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Oh Chang Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Jeong Dae Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut Pediatric Cardiology Springer-Verlag 32/7(2011-10-01), 947-952 0172-0643 32:7<947 2011 32 246 https://doi.org/10.1007/s00246-011-0019-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00246-011-0019-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moon Se Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 700 1- Han Ji Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 700 1- Hwang Hui Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Mee Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Soon Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Jae Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 700 1- Oh Chang Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 700 1- Jeong Dae Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea aut NATIONALLICENCE 773 0- Pediatric Cardiology Springer-Verlag 32/7(2011-10-01), 947-952 0172-0643 32:7<947 2011 32 246 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer