caa a22 4500 445831480 CHVBK 20180317145316.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00586-010-1619-z doi (NATIONALLICENCE)springer-10.1007/s00586-010-1619-z Differential response of human bone marrow stromal cells to either TGF-β1 or rhGDF-5 [Elektronische Daten] [Benjamin Gantenbein-Ritter, Lorin Benneker, Mauro Alini, Sibylle Grad] Cell therapy along with growth factor injection is currently widely investigated to restore the intervertebral disc. However, there is increasing evidence that transplanted unconditioned bone marrow-derived stromal cells (BMSCs) cannot thrive in the intervertebral disc "niche”. Moreover, uncertainty exists with respect to the cell phenotype that would be suitable to inject. The intervertebral disc cell phenotype only recently has been started to be characterised using transcriptomics profiling. Recent findings suggest that cytokeratin 19 (KRT-19) could be used as a potential candidate marker for the intervertebral disc, or more specifically the nucleus pulposus cell (NPC) phenotype. We present in vitro cell culture data using alginate bead culture of primary human BMSCs exposed to the standard chondrogenic stimulus, transforming growth factor beta-1 (TGF-β), the growth and differentiation factor 5 and/or bovine NPCs to induce a potential "discogenic” pathway. Chondrogenic induction via TGF-β pathway provoked down-regulation of KRT-19 gene expression in four out of five donors after 18days of culture, whereas KRT-19 expression remained unchanged in the "discogenic” groups. In addition, the ratio of aggrecan/collagen II gene expression showed a remarkable difference (of at least 3 magnitudes) between the chondrogenic stimulus (low ratio) and the discogenic stimulus (high ratio). Therefore, KRT-19 and aggrecan/collagen II ratio may be potential markers to distinguish chondrogenic from "discogenic” differentiation. Springer-Verlag, 2010 Coculture nationallicence Bone marrow stromal cells nationallicence Growth factor rhGDF-5 nationallicence CDMP-1 nationallicence Bovine nucleus pulposus cells nationallicence Gene expression nationallicence Differentiation nationallicence Gantenbein-Ritter Benjamin AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland aut Benneker Lorin Insel University Hospital, Bern, Switzerland aut Alini Mauro AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland aut Grad Sibylle AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland aut European Spine Journal Springer-Verlag 20/6(2011-06-01), 962-971 0940-6719 20:6<962 2011 20 586 https://doi.org/10.1007/s00586-010-1619-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-010-1619-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gantenbein-Ritter Benjamin AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland aut NATIONALLICENCE 700 1- Benneker Lorin Insel University Hospital, Bern, Switzerland aut NATIONALLICENCE 700 1- Alini Mauro AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland aut NATIONALLICENCE 700 1- Grad Sibylle AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/6(2011-06-01), 962-971 0940-6719 20:6<962 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer