caa a22 4500 445831855 CHVBK 20180317145317.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00586-011-1767-9 doi (NATIONALLICENCE)springer-10.1007/s00586-011-1767-9 Mechanical strain induces Cx43 expression in spinal ligament fibroblasts derived from patients presenting ossification of the posterior longitudinal ligament [Elektronische Daten] [Hai-song Yang, Xu-hua Lu, De-yu Chen, Wen Yuan, Li-li Yang, Yu Chen, Hai-long He] Ossification of the posterior longitudinal ligament (OPLL) is characterized by ectopic bone formation in spinal ligaments. Some evidence indicates that mechanical strain can lead to the development of OPLL, although the signaling mechanism is not fully understood. Connexin43 (Cx43), a gap-junction protein, has been shown to be of particular importance in bone formation. We hypothesized that Cx43 may play an important role in the signal transmission induced by mechanical strain during the development of OPLL. To explore this possibility, we cultured fibroblasts from spinal ligaments of OPLL and non-OPLL patients and preloaded mechanical stretch onto the cells via a Flexercell 4000 Tension Plus system. We evaluated expression changes in osteocalcin (OCN), alkaline phosphatase (ALP), type I collagen (COL I) and Cx43 via semi-quantitative RT-PCR and western blotting at 12 and 24h after mechanical strain application in contrast to static conditions. We observed a significant gene up-regulation of OCN, ALP and COL I and Cx43 protein in OPLL cells after mechanical strain application, but no changes in non-OPLL cells. Notably, after RNA interference targeting Cx43 was performed in OPLL cells, we found that there were no significant changes in the expressions of OCN, ALP, COL I and Cx43 after the mechanical strain was applied for 24h. Thus, we propose that the increase in Cx43 expression induced by mechanical strain in OPLL cells plays an important role in the progression of OPLL. Springer-Verlag, 2011 Ossification nationallicence Posterior longitudinal ligament nationallicence Mechanical stress nationallicence Connexin43 nationallicence Gap junction nationallicence Yang Hai-song Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut Lu Xu-hua Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut Chen De-yu Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut Yuan Wen Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut Yang Li-li Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut Chen Yu Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut He Hai-long Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut European Spine Journal Springer-Verlag 20/9(2011-09-01), 1459-1465 0940-6719 20:9<1459 2011 20 586 https://doi.org/10.1007/s00586-011-1767-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-011-1767-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Hai-song Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut NATIONALLICENCE 700 1- Lu Xu-hua Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut NATIONALLICENCE 700 1- Chen De-yu Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut NATIONALLICENCE 700 1- Yuan Wen Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut NATIONALLICENCE 700 1- Yang Li-li Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut NATIONALLICENCE 700 1- Chen Yu Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut NATIONALLICENCE 700 1- He Hai-long Department of Orthopaedic Surgery, Changzheng Hospital, No. 415 Feng Yang Road, 200003, Shanghai, China aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/9(2011-09-01), 1459-1465 0940-6719 20:9<1459 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer