caa a22 4500 445832797 CHVBK 20180317145320.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00586-010-1664-7 doi (NATIONALLICENCE)springer-10.1007/s00586-010-1664-7 In vitro evaluation of a manganese chloride phantom-based MRI technique for quantitative determination of lumbar intervertebral disc composition and condition [Elektronische Daten] [Lachlan Smith, Andrew Kurmis, John Slavotinek, Nicola Fazzalari] The application of MRI as a non-invasive, quantitative tool for diagnosing lumbar intervertebral disc degeneration is currently an area of active research. The objective of this study was to examine, in vitro, the efficacy of a manganese chloride phantom-based MRI technique for quantitatively assessing lumbar disc composition and degenerative condition. Sixteen human lumbar discs were imaged ex vivo using T2-weighted MRI, and assigned a quantitative grade based on the relative signal intensities of nine phantoms containing serial concentrations of manganese chloride. Discs were then graded macroscopically for degenerative condition, and water and uronic acid (glycosaminoglycan) contents were determined. MRI ranking exhibited significant and strong negative correlation with nucleus pulposus uronic acid content (r=−0.78). MRI grades were significantly higher for degenerate discs. The technique described presents immediate potential for in vitro studies requiring robust, minimally invasive and quantitative determination of lumbar disc composition and condition. Additionally, the technique may have potential as a clinical tool for diagnosing lumbar disc degeneration as it provides a standardised series of reference phantoms facilitating cross-platform consistency, requires short scan times and simple T2-weighted signal intensity measurements. Springer-Verlag, 2010 Intervertebral disc nationallicence Disc composition nationallicence Disc condition nationallicence MRI nationallicence Manganese chloride nationallicence Smith Lachlan Bone and Joint Research Laboratory, Surgical Pathology SA Pathology, Frome Rd, 5000, Adelaide, SA, Australia aut Kurmis Andrew Bone and Joint Research Laboratory, Surgical Pathology SA Pathology, Frome Rd, 5000, Adelaide, SA, Australia aut Slavotinek John Division of Medical Imaging, Flinders Medical Centre, 5042, Bedford Park, SA, Australia aut Fazzalari Nicola Bone and Joint Research Laboratory, Surgical Pathology SA Pathology, Frome Rd, 5000, Adelaide, SA, Australia aut European Spine Journal Springer-Verlag 20/3(2011-03-01), 434-439 0940-6719 20:3<434 2011 20 586 https://doi.org/10.1007/s00586-010-1664-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-010-1664-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith Lachlan Bone and Joint Research Laboratory, Surgical Pathology SA Pathology, Frome Rd, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Kurmis Andrew Bone and Joint Research Laboratory, Surgical Pathology SA Pathology, Frome Rd, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 700 1- Slavotinek John Division of Medical Imaging, Flinders Medical Centre, 5042, Bedford Park, SA, Australia aut NATIONALLICENCE 700 1- Fazzalari Nicola Bone and Joint Research Laboratory, Surgical Pathology SA Pathology, Frome Rd, 5000, Adelaide, SA, Australia aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/3(2011-03-01), 434-439 0940-6719 20:3<434 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer