caa a22 4500 445833769 CHVBK 20180627064141.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00586-010-1662-9 doi (NATIONALLICENCE)springer-10.1007/s00586-010-1662-9 Human mesenchymal stem cell co-culture modulates the immunological properties of human intervertebral disc tissue fragments in vitro [Elektronische Daten] [Alessandro Bertolo, Thomas Thiede, Niklaus Aebli, Martin Baur, Stephen Ferguson, Jivko Stoyanov] The capacity of mesenchymal stem cells (MSCs) to differentiate into intervertebral disc (IVD)-like cells has been well described, but their ability to modulate the inflammatory processes in the IVD remains unclear. We found that tissue obtained by discectomy of degenerated and post-traumatic IVD contains significant amounts of IgG antibodies, a sign of lymphocyte infiltration. Further we investigated whether MSCs in vitro, which were characterized for their multilineage differentiation potential and may have immunomodulatory effects on IVD fragments. IVD fragments were co-cultured in contact with peripheral blood lymphocytes (PBLs) and MSCs, and as functional controls we used contact co-cultures of PBLs stimulated with pokeweed mitogen (2.5μg/mL) and MSCs. The time course of lymphocyte proliferation (Alamar Blue), IgG (ELISA) and gene expression (RT-PCR) of anti-inflammatory cytokines (TGF-β1, IL-10) by MSCs and pro-inflammatory molecules (IL-1α, IL-1β and TNF-α) by the IVD fragments were analyzed. Depending on the response to the presence of MSCs, the IVD fragments (n=13) were divided in two groups: responders (n=9), where inflammation was inhibited by MSCs and non-responders (n=4), where MSCs did not decrease inflammation. At 1week in co-culture, MSCs reduced significantly the IgG production in the IVD responders group to 69% and PBLs proliferation to 57% of the control. MSCs expression of the anti-inflammatory TGF-β1 increased with time, while IL-10 was expressed only at day 1. IVD gene expression of TNF-α decreased constantly, whereas IL-1α and IL-1β expression increased. In conclusion, these data suggest that MSCs may modulate disc-specific inflammatory and pain status and aid regeneration of the host tissue. Springer-Verlag, 2010 Mesenchymal stem cells nationallicence Intervertebral discs nationallicence Peripheral blood lymphocytes nationallicence Immunomodulation nationallicence Bertolo Alessandro Swiss Paraplegic Research, G.A. Zäch Str. 4, Nottwil, Switzerland aut Thiede Thomas Swiss Paraplegic Center, Nottwil, Switzerland aut Aebli Niklaus Swiss Paraplegic Center, Nottwil, Switzerland aut Baur Martin Cantonal Hospital of Luzern, Lucerne, Switzerland aut Ferguson Stephen Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland aut Stoyanov Jivko Swiss Paraplegic Research, G.A. Zäch Str. 4, Nottwil, Switzerland aut European Spine Journal Springer-Verlag 20/4(2011-04-01), 592-603 0940-6719 20:4<592 2011 20 586 https://doi.org/10.1007/s00586-010-1662-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-010-1662-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bertolo Alessandro Swiss Paraplegic Research, G.A. Zäch Str. 4, Nottwil, Switzerland aut NATIONALLICENCE 700 1- Thiede Thomas Swiss Paraplegic Center, Nottwil, Switzerland aut NATIONALLICENCE 700 1- Aebli Niklaus Swiss Paraplegic Center, Nottwil, Switzerland aut NATIONALLICENCE 700 1- Baur Martin Cantonal Hospital of Luzern, Lucerne, Switzerland aut NATIONALLICENCE 700 1- Ferguson Stephen Institute for Surgical Technology and Biomechanics, University of Bern, Bern, Switzerland aut NATIONALLICENCE 700 1- Stoyanov Jivko Swiss Paraplegic Research, G.A. Zäch Str. 4, Nottwil, Switzerland aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/4(2011-04-01), 592-603 0940-6719 20:4<592 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 445833769 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer