caa a22 4500 445834196 CHVBK 20180317145325.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00586-011-1721-x doi (NATIONALLICENCE)springer-10.1007/s00586-011-1721-x Development of an intact intervertebral disc organ culture system in which degeneration can be induced as a prelude to studying repair potential [Elektronische Daten] [Bernice Jim, Thomas Steffen, Janet Moir, Peter Roughley, Lisbet Haglund] The present work describes a novel bovine disc organ culture system with long-term maintenance of cell viability, in which degenerative changes can be induced as a prelude to studying repair. Discs were isolated with three different techniques: without endplates (NEP), with bony endplates (BEP) and with intact cartilage endplates (CEP). Swelling, deformation, and cell viability were evaluated in unloaded cultures. Degeneration was induced by a single trypsin injection into the center of the disc and the effect on cell viability and matrix degradation was followed. Trypsin-treated discs were exposed to TGFβ to evaluate the potential to study repair in this system. NEP isolated discs showed >75% maintained cell viability for up to 10days but were severely deformed, BEP discs on the other hand maintained morphology but failed to retain cell viability having only 27% viable cells after 10days. In CEP discs, both cell viability and morphology were maintained for at least 4weeks where >75% of the cells were still viable. To mimic proteoglycan loss during disc degeneration, a single trypsin injection was administered to the center of the disc. This resulted in 60% loss of aggrecan, after 7days, without affecting cell viability. When TGFβ was injected to validate that the system can be used to study a repair response following injection of a bio-active substance, proteoglycan synthesis nearly doubled compared to baseline synthesis. Trypsin-treated bovine CEP discs therefore provide a model system for studying repair of the degenerate disc, as morphology, cell viability and responsiveness to bio-active substances were maintained. Springer-Verlag, 2011 Intervertebral disc nationallicence Degeneration nationallicence Repair nationallicence Organ culture nationallicence Jim Bernice Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut Steffen Thomas Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut Moir Janet Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut Roughley Peter Shriners Hospital for Children, McGill University, 1529 Cedar Avenue, H3G 1AG, Montreal, QC, Canada aut Haglund Lisbet Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut European Spine Journal Springer-Verlag 20/8(2011-08-01), 1244-1254 0940-6719 20:8<1244 2011 20 586 https://doi.org/10.1007/s00586-011-1721-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-011-1721-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jim Bernice Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Steffen Thomas Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Moir Janet Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Roughley Peter Shriners Hospital for Children, McGill University, 1529 Cedar Avenue, H3G 1AG, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Haglund Lisbet Orthopaedic Research Laboratory, McGill University, 687 Pine Avenue West, Room L4.70, H3A 1A1, Montreal, QC, Canada aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/8(2011-08-01), 1244-1254 0940-6719 20:8<1244 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer