caa a22 4500 445834722 CHVBK 20180317145327.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00586-011-1901-8 doi (NATIONALLICENCE)springer-10.1007/s00586-011-1901-8 Titanium and tantalum as mesenchymal stem cell scaffolds for spinal fusion: an in vitro comparative study [Elektronische Daten] [Juan Blanco, Fermin Sánchez-Guijo, Soraya Carrancio, Sandra Muntion, Jesus García-Briñon, Maria-Consuelo del Cañizo] Introduction: In the last few years, great interest has been focused on tissue engineering as a potential therapeutic approach for musculoskeletal diseases. The role of metallic implants for spinal fusion has been tested in preclinical and clinical settings. Titanium and tantalum have excellent biocompatibility and mechanical properties and are being used in this situation. On the other hand, the therapeutic role of mesenchymal stem cells (MSC) is extensively explored for their multilineage differentiation into osteoblasts. Objetives: In vitro comparision of titanium and tantalum as MSCs scaffolds. Material and methods: In the present study, we have compared the in vitro expansion capacity, viability, immunophenotype (both explored by flow cytometry) and multi-differentiation ability of MSC cultured in the presence of either titanium or tantalum fragments. The adherence of MSC to either metal was demonstrated by electron microscopy. Results: Both metals were able to carry MSC when transferred to new culture flasks. In addition, our study shows that culture of MSC with titanium or tantalum improves cell viability and maintains all their biological properties, with no significant differences regarding the metal employed. Conclusion: This would support the use of these combinations for clinical purposes, especially in the spinal fusion and reconstruction setting. Springer-Verlag, 2011 Titanium scaffold nationallicence Tantalum scaffold nationallicence Mesenchymal stem cells nationallicence Tissue engineering nationallicence Blanco Juan Department of Orthopedics, Servicio de Traumatología y CO, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut Sánchez-Guijo Fermin Department of Hematology, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut Carrancio Soraya Cell Therapy Lab, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut Muntion Sandra Cell Therapy Lab, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut García-Briñon Jesus Department of Cell Biology, Facultad de Medicina, Universidad de Salamanca, Salamanca, Spain aut del Cañizo Maria-Consuelo Department of Hematology, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut European Spine Journal Springer-Verlag 20(2011-08-01), 353-360 0940-6719 20<353 2011 20 586 https://doi.org/10.1007/s00586-011-1901-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-011-1901-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Blanco Juan Department of Orthopedics, Servicio de Traumatología y CO, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Sánchez-Guijo Fermin Department of Hematology, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Carrancio Soraya Cell Therapy Lab, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- Muntion Sandra Cell Therapy Lab, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut NATIONALLICENCE 700 1- García-Briñon Jesus Department of Cell Biology, Facultad de Medicina, Universidad de Salamanca, Salamanca, Spain aut NATIONALLICENCE 700 1- del Cañizo Maria-Consuelo Department of Hematology, Hospital Universitario de Salamanca, Paseo de San Vicente 58, 37007, Salamanca, Spain aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20(2011-08-01), 353-360 0940-6719 20<353 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer