caa a22 4500 445835044 CHVBK 20180317145328.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00586-011-1736-3 doi (NATIONALLICENCE)springer-10.1007/s00586-011-1736-3 Autografts for spinal fusion: osteogenic potential of laminectomy bone chips and bone shavings collected via high speed drill [Elektronische Daten] [Claudia Eder, Albert Chavanne, Jochen Meissner, Wolfgang Bretschneider, Alexander Tuschel, Philipp Becker, Michael Ogon] In case of revision or minimal invasive spinal surgery, the amount of autograft possibly harvested from the lamina and the spinous processes is limited. Ekanayake and Shad (Acta Neurochir 152:651-653, 2010) suggest the application of bone shavings harvested via high speed burr additionally or instead, but so far no data regarding their osteogenic potential exist. Aim of the study was to compare the osteogenic potential of bone chips and high speed burr shavings, and to evaluate the applicability of bone shavings as an autograft for spinal fusion. Bone chips and shavings from 14 patients undergoing spinal decompression surgery were analyzed using in vitro tissue culture methods. Osteoblast emigration and proliferation, viability and mineralization were investigated and histological evaluation was performed. Bone chips from all patients showed successful osteoblast emigration after average 5.5days. In contrast, only 57% of the corresponding bone shavings successfully demonstrated osteoblast emigration within an average time span of 14.8days. Average osteoblast mobilisation was 1.25×106cells per gram from bone chips and 1.73×105cells per gram from the corresponding bone shavings. No difference was observed regarding cell viability, but population doubling times of bone chip cultures were significantly lower (50.5 vs. 121h) and mineralization was observed in osteoblasts derived from bone chips only. Although some authors suggest the general applicability of laminectomy bone shavings as autografts for spinal fusion, autologous bone grafts obtained from laminectomy bone chips are superior in terms of cell delivery, cell proliferation and mineralization. Springer-Verlag, 2011 Spinal fusion nationallicence Bone graft nationallicence Osteoregeneration nationallicence Bone chips nationallicence High speed burr nationallicence Eder Claudia 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut Chavanne Albert 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut Meissner Jochen 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut Bretschneider Wolfgang 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut Tuschel Alexander 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut Becker Philipp 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut Ogon Michael 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut European Spine Journal Springer-Verlag 20/11(2011-11-01), 1791-1795 0940-6719 20:11<1791 2011 20 586 https://doi.org/10.1007/s00586-011-1736-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-011-1736-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eder Claudia 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut NATIONALLICENCE 700 1- Chavanne Albert 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut NATIONALLICENCE 700 1- Meissner Jochen 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut NATIONALLICENCE 700 1- Bretschneider Wolfgang 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut NATIONALLICENCE 700 1- Tuschel Alexander 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut NATIONALLICENCE 700 1- Becker Philipp 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut NATIONALLICENCE 700 1- Ogon Michael 3rd Orthopedic Department, Orthopedic Hospital Vienna-Speising, Speisinger Str. 109, 1130, Vienna, Austria aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/11(2011-11-01), 1791-1795 0940-6719 20:11<1791 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer