caa a22 4500 445835052 CHVBK 20180317145328.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00586-011-1877-4 doi (NATIONALLICENCE)springer-10.1007/s00586-011-1877-4 The effect of repeated restraint stress in pain-related behavior induced by nucleus pulposus applied on the nerve root in rats [Elektronische Daten] [Kazuhide Uesugi, Miho Sekiguchi, Shin-ichi Kikuchi, Shin-ichi Konno] Introduction: Chronic pain has an impact on psychological and social factors. It is known that stress influences physiological and behavioral changes and affects several neurotransmitter and hormonal systems. It is also known that corticosterone is increased by stress. The role of chronic stress in sciatica in lumbar disc herniation (LDH) in rats has not been investigated. The aim of this study was to investigate the effect of the restraint stress (RS) on pain-related behavior induced by application of nucleus pulposus (NP) in rats. Materials and methods: Adult female Sprague-Dawley rats were divided into six experimental groups (naive group; naive+RS; sham group; sham+RS; autologous nucleus pulposus [NP] applied on the left L5 nerve root [NP group]; and NP+RS group). Von Frey tests were used to test pain-related behavior. Concentrations of plasma corticosterone were measured to assess changes in levels of endogenous corticosterone caused by RS. Expression of ATF-3 in the left L5 DRG was examined by immunohistochemical analyses in each group. Results: Mechanical withdrawal thresholds of the NP and NP+RS groups were significantly decreased after surgery compared with the naive group. Although the thresholds in the NP group recovered after 28days, the thresholds in the NP+RS group were significantly decreased during the 42days after surgery. RS increased the concentration of plasma corticosterone at 21 and 42days after surgery. In the NP and the NP+RS groups, the expression of ATF-3 was significantly increased at 7days after surgery. The expression of ATF-3 was sustained for 21days by RS. Conclusion: Concentrations of plasma corticosterone were increased in three groups that underwent RS. The pain-related behavior persisted for the long term in the LDH model. The expression of ATF-3 in DRG neurons increased for 21days by RS. These results suggest that RS plays a role in the chronicity of pain-related behavior in the LDH rats. Springer-Verlag, 2011 Repeated restraint stress nationallicence Corticosterone nationallicence Pain-related behavior nationallicence Nucleus pulposus nationallicence Lumbar disc herniation nationallicence Uesugi Kazuhide Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut Sekiguchi Miho Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut Kikuchi Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut Konno Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut European Spine Journal Springer-Verlag 20/11(2011-11-01), 1885-1891 0940-6719 20:11<1885 2011 20 586 https://doi.org/10.1007/s00586-011-1877-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-011-1877-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Uesugi Kazuhide Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut NATIONALLICENCE 700 1- Sekiguchi Miho Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut NATIONALLICENCE 700 1- Kikuchi Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut NATIONALLICENCE 700 1- Konno Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1-Hikarigaoka, 960-1295, Fukushima City, Fukushima, Japan aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/11(2011-11-01), 1885-1891 0940-6719 20:11<1885 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer