caa a22 4500 445835141 CHVBK 20180317145328.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00586-011-1854-y doi (NATIONALLICENCE)springer-10.1007/s00586-011-1854-y Etanercept attenuates pain-related behavior following compression of the dorsal root ganglion in the rat [Elektronische Daten] [Kazuyuki Watanabe, Shoji Yabuki, Miho Sekiguchi, Shin-ichi Kikuchi, Shin-ichi Konno] Purpose: TNFα is an inflammatory mediator related to neuropathic pain including sciatica. Much basic research suggests that anti-TNFα therapy may be useful for the treatment of sciatica. The purpose of this study was to clarify the effects of etanercept in a dorsal root ganglion (DRG) compression model. Methods: Adult male Sprague-Dawley rats (200-250g, n=60) were used. An L-shaped stainless rod was used to compress the left L5 DRG in the saline and etanercept groups. No rod was used in the sham group. In the etanercept group, 1mg of etanercept was applied locally onto the DRG at the end of surgery. Saline was applied in the saline and sham groups. On day 3 and day 7 after surgery, the number of ED1-immunoreactive (IR) cells (macrophages) in the DRG was calculated by immunohistochemical methods (n=6). In addition, double-immunofluorescence labeling for ED1 and TNFα was performed. Behavioral testing with von Frey filaments and a heat stimulator was performed (n=12). Results: ED1-IR cells in the DRG significantly increased in the control group compared with the sham group (p<0.05). Some ED1-IR cells were co-labeled for TNFα. In the etanercept group, decrease in mechanical threshold was significantly inhibited compared with the saline group (p<0.05). Thermal hyperalgesia was observed in the control group, but in neither the sham nor etanercept group (p<0.05). Conclusion: Etanercept attenuated the pain-related behavior induced by DRG compression. These findings suggest that mechanical effects on the DRG might be reduced by etanercept in addition to the effects on nucleus pulposus in lumbar disc herniation. Springer-Verlag, 2011 Sciatica nationallicence Dorsal root ganglion nationallicence Tumor necrosis factor-α nationallicence Etanercept nationallicence Macrophage nationallicence Watanabe Kazuyuki Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut Yabuki Shoji Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut Sekiguchi Miho Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut Kikuchi Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut Konno Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut European Spine Journal Springer-Verlag 20/11(2011-11-01), 1877-1884 0940-6719 20:11<1877 2011 20 586 https://doi.org/10.1007/s00586-011-1854-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00586-011-1854-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Watanabe Kazuyuki Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut NATIONALLICENCE 700 1- Yabuki Shoji Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut NATIONALLICENCE 700 1- Sekiguchi Miho Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut NATIONALLICENCE 700 1- Kikuchi Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut NATIONALLICENCE 700 1- Konno Shin-ichi Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, 1 Hikarigaoka, 960-1295, Fukushima, Fukushima, Japan aut NATIONALLICENCE 773 0- European Spine Journal Springer-Verlag 20/11(2011-11-01), 1877-1884 0940-6719 20:11<1877 2011 20 586 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer