caa a22 4500 445840188 CHVBK 20180317145341.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00709-010-0251-4 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0251-4 Plasmodesmal receptor-like kinases identified through analysis of rice cell wall extracted proteins [Elektronische Daten] [Yeonhwa Jo, Won Cho, Yeonggil Rim, Juyeon Moon, Xiong-Yan Chen, Hyosub Chu, Cha Kim, Zee-Yong Park, William Lucas, Jae-Yean Kim] In plants, plasmodesmata (PD) are intercellular channels that function in both metabolite exchange and the transport of proteins and RNAs. Currently, many of the PD structural and regulatory components remain to be elucidated. Receptor-like kinases (RLKs) belonging to a notably expanded protein family in plants compared to the animal kingdom have been shown to play important roles in plant growth, development, pathogen resistance, and cell death. In this study, cell biological approaches were used to identify potential PD-associated RLK proteins among proteins contained within cell walls isolated from rice callus cultured cells. A total of 15 rice RLKs were investigated to determine their subcellular localization, using an Agrobacterium-mediated transient expression system. Of these six PD-associated RLKs were identified based on their co-localization with a viral movement protein that served as a PD marker, plasmolysis experiments, and subcellular localization at points of wall contact between spongy mesophyll cells. These findings suggest potential PD functions in apoplasmic signaling in response to environmental stimuli and developmental inputs. The Author(s), 2010 Plasmodesmata nationallicence Receptor-like kinase nationallicence Symplasmic signaling nationallicence Apoplasmic signaling nationallicence Cell wall proteomics nationallicence PD : Plasmodesmata nationallicence PM : Plasma membrane nationallicence ER : Endoplasmic reticulum nationallicence MP : Movement protein nationallicence RLK : Receptor-like kinase nationallicence Jo Yeonhwa Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Cho Won Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Rim Yeonggil Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Moon Juyeon Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Chen Xiong-Yan Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Chu Hyosub Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Kim Cha Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Park Zee-Yong Department of Life Science, Gwangju Institute of Science and Technology, 500-712, Gwangju, Republic of Korea aut Lucas William Department of Plant Biology, College of Biological Sciences, University of California, 95616, Davis, CA, USA aut Kim Jae-Yean Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut Protoplasma Springer Vienna 248/1(2011-01-01), 191-203 0033-183X 248:1<191 2011 248 709 https://doi.org/10.1007/s00709-010-0251-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0251-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jo Yeonhwa Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Cho Won Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Rim Yeonggil Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Moon Juyeon Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Chen Xiong-Yan Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Chu Hyosub Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Kim Cha Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 700 1- Park Zee-Yong Department of Life Science, Gwangju Institute of Science and Technology, 500-712, Gwangju, Republic of Korea aut NATIONALLICENCE 700 1- Lucas William Department of Plant Biology, College of Biological Sciences, University of California, 95616, Davis, CA, USA aut NATIONALLICENCE 700 1- Kim Jae-Yean Division of Applied Life Science (BK21 Program), Environmental Biotechnology National Core Research Center, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Republic of Korea aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/1(2011-01-01), 191-203 0033-183X 248:1<191 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer