caa a22 4500 44584034X CHVBK 20180317145342.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00709-010-0216-7 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0216-7 Constitutive expression of high-affinity sulfate transporter (HAST) gene in Indian mustard showed enhanced sulfur uptake and assimilation [Elektronische Daten] [M. Abdin, M. Akmal, M. Ram, T. Nafis, P. Alam, M. Nadeem, M. Khan, A. Ahmad] Lycopersicon esculantum sulfate transporter gene (LeST 1.1) encodes a high-affinity sulfate transporter (HAST) located in root epidermis. In this study, the LeST 1.1 gene was constitutively expressed in Indian mustard (Brassica juncea cv. Pusa Jai Kisan). Transgenic as well as untransformed plants were grown in sulfur-insufficient (25 and 50μM) and sulfur-sufficient (1,000μM) conditions for 30days. Two-fold increase was noticed in the sulfate uptake rate of transgenic plants grown in both sulfur-insufficient and -sufficient conditions as compared to untransformed plants. The transgenic B. juncea plants were able to accumulate higher biomass and showed improved sulfur status even in sulfur-insufficient conditions when compared with untransformed plants. Chlorophyll content, ATP sulfurylase activity and protein content were also higher in transgenic plants than untranformed plants under sulfur-insufficient conditions. Our results, thus, clearly indicate that constitutive expression of LeST 1.1 gene in B. juncea had led to enhanced capacity of sulfur uptake and assimilation even in sulfur-insufficient conditions. This approach can also be used in other crops to enhance their sulfate uptake and assimilation potential under S-insufficient conditions. Springer-Verlag, 2010 Brassica juncea (L.) Czern. and Coss nationallicence Transgenic plants nationallicence Genetic transformation nationallicence Lycopersicon esculantum L nationallicence Sulfate transporter nationallicence Sulfate uptake nationallicence Abdin M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Akmal M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Ram M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Nafis T. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Alam P. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Nadeem M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Khan M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Ahmad A. Department of Botany, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut Protoplasma Springer Vienna 248/3(2011-07-01), 591-600 0033-183X 248:3<591 2011 248 709 https://doi.org/10.1007/s00709-010-0216-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0216-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abdin M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Akmal M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Ram M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Nafis T. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Alam P. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Nadeem M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Khan M. Department of Biotechnology, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Ahmad A. Department of Botany, Faculty of Science, Jamia Hamdard, 110062, New Delhi, India aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/3(2011-07-01), 591-600 0033-183X 248:3<591 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer