caa a22 4500 445840358 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00709-010-0210-0 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0210-0 Arsenite treatment induces oxidative stress, upregulates antioxidant system, and causes phytochelatin synthesis in rice seedlings [Elektronische Daten] [Shruti Mishra, A. Jha, R. Dubey] The effects of arsenite treatment on generation of reactive oxygen species, induction of oxidative stress, response of antioxidative system, and synthesis of phytochelatins were investigated in two indica rice (Oryza sativa L.) cvs. Malviya-36 and Pant-12 grown in sand cultures for a period of 5-20days. Arsenite (As2O3; 25 and 50μM) treatment resulted in increased formation of superoxide anion (O 2 .− ), elevated levels of H2O2 and thiobarbituric acid reactive substances, showing enhanced lipid peroxidation. An enhanced level of ascorbate (AA) and glutathione (GSH) was observed irrespective of the variation in the level of dehydroascorbate (DHA) and oxidized glutathione (GSSG) which in turn influenced redox ratios AA/DHA and GSH/GSSG. With progressive arsenite treatment, synthesis of total acid soluble thiols and phytochelatins (PC) increased in the seedlings. Among antioxidative enzymes, the activities of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6), total ascorbate peroxidase (APX, EC 1.11.1.11), chloroplastic ascorbate peroxidase, guaiacol peroxidase (EC 1.11.1.7), monodehydroascorbate reductase (EC 1.6.5.4), and glutathione reductase (EC 1.6.4.2) increased in arsenite treated seedlings, while dehyroascorbate reductase (EC 1.8.5.1) activity declined initially during 5-10days and increased thereafter. Results suggest that arsenite treatment causes oxidative stress in rice seedlings, increases the levels of many enzymatic and non-enzymatic antioxidants, and induces synthesis of thiols and PCs, which may serve as important components in mitigating arsenite-induced oxidative damage. Springer-Verlag, 2010 Antioxidants nationallicence Antioxidative enzymes nationallicence Arsenite nationallicence Lipid peroxides nationallicence Reactive oxygen species nationallicence Phytochelatin nationallicence Rice nationallicence Oryza sativa L nationallicence Mishra Shruti Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Jha A. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Dubey R. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut Protoplasma Springer Vienna 248/3(2011-07-01), 565-577 0033-183X 248:3<565 2011 248 709 https://doi.org/10.1007/s00709-010-0210-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0210-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mishra Shruti Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 700 1- Jha A. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 700 1- Dubey R. Department of Biochemistry, Faculty of Science, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/3(2011-07-01), 565-577 0033-183X 248:3<565 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer