caa a22 4500 445840374 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00709-010-0188-7 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0188-7 Wen Bin Key Laboratory of Tropical Forest Ecology, Xishuangbanana Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Mengla, Yunnan, China aut Cytological and physiological changes related to cryotolerance in recalcitrant Livistona chinensis embryos during seed development [Elektronische Daten] [Bin Wen] Cytological and biochemical changes in recalcitrant Livistona chinensis embryos following the acquisition and loss of cryotolerance to liquid nitrogen during seed development were studied. The embryonic cells were always hydrated and contained fully functional organelles throughout seed development. However, the central cells in the root-epicotyl end of the embryo exhibited partial dedifferentiation during the middle developmental stages, although extensive reduction of mitochondria and vacuolation and intensive accumulation of starch grains, lipid, and protein bodies were not observed. Total soluble sugar content rose then decreased on a fresh weight and water weight basis, while soluble and heat-stable proteins increased in number and content then decreased, as seeds matured. These cytological and biochemical features differ from those of orthodox seeds, providing a physiological basis for the recalcitrant behavior of L. chinensis seeds. The changes were closely correlated with acquisition and loss of cryotolerance in L. chinensis embryos and are presumed to contribute to cryotolerance, which would account for the cryotolerance variation in L. chinensis embryos. Cryotolerance is suggested to be a complex, multifaceted process, and accumulation of soluble sugars and soluble and heat-stable proteins alone is not enough to increase cryotolerance per se without acting in combination with a decrease of cellular metabolic activity. Springer-Verlag, 2010 Chinese fan palm nationallicence Cryopreservation nationallicence Cryotolerance nationallicence Desiccation tolerance nationallicence Livistona chinensis nationallicence Recalcitrant seeds nationallicence Seed development nationallicence WAF : Weeks after flowering nationallicence W50FS : The upper limit of water content allowing 50% post-thaw embryos to survive nationallicence Protoplasma Springer Vienna 248/3(2011-07-01), 483-491 0033-183X 248:3<483 2011 248 709 https://doi.org/10.1007/s00709-010-0188-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0188-7 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Wen Bin Key Laboratory of Tropical Forest Ecology, Xishuangbanana Tropical Botanical Garden, Chinese Academy of Sciences, 666303, Mengla, Yunnan, China aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/3(2011-07-01), 483-491 0033-183X 248:3<483 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 445840374 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer