caa a22 4500 445840382 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00709-010-0204-y doi (NATIONALLICENCE)springer-10.1007/s00709-010-0204-y Roles of arabinogalactan proteins in cotyledon formation and cell wall deposition during embryo development of Arabidopsis [Elektronische Daten] [Jing Zhong, YuJun Ren, Miao Yu, TengFei Ma, XueLian Zhang, Jie Zhao] Arabinogalactan proteins (AGPs) are a class of highly glycosylated, widely distributed proteins in higher plants. In the previous study, we found that the green fluorescence from JIM13-labeled AGPs was mainly distributed in embryo proper and the basal part of suspensor but gradually disappeared after the torpedo-stage embryos in Arabidopsis. And (β-d-Glc)3 Yariv phenylglycoside (βGlcY), a synthetic reagent that specifically binds to AGPs, could inhibit embryo development. In this study, as a continuous work, we investigated the AGP functions in embryo germination, cotyledon formation, and cell wall deposition in Arabidopsis embryos by using immunofluorescent, immunoenzyme, transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR) techniques. The results showed that 50 μM βGlcY caused inhibition of embryo germination, formation of abnormal cotyledon embryos, and disorder of cotyledon vasculature. Compared with the normal embryos in vitro and in vivo, the AGPs and pectin signals were quite weaker in the whole abnormal embryos, whereas the cellulose signal was stronger in the shoot apical meristem (SAM) of abnormal embryo by calcofluor white staining. The FTIR assay demonstrated that the cell wall of abnormal embryos was relatively poorer in pectins and richer in cellulose than those of normal embryos. By TEM observation, the SAM cells of the abnormal embryos had less cytoplasm, more plastid and starch grains, and larger vacuole than that of normal embryos. These results indicated that AGPs may play roles in embryo germination, cotyledon formation, cell wall cellulose and pectin deposition, and cell division potentiality during embryo development of Arabidopsis. Springer-Verlag, 2010 Arabidopsis thaliana nationallicence Arabinogalactan proteins nationallicence Cotyledon nationallicence Cellulose nationallicence Pectin nationallicence Shoot apical meristem nationallicence Zhong Jing Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Ren YuJun Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Yu Miao Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Ma TengFei Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Zhang XueLian Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Zhao Jie Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut Protoplasma Springer Vienna 248/3(2011-07-01), 551-563 0033-183X 248:3<551 2011 248 709 https://doi.org/10.1007/s00709-010-0204-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0204-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhong Jing Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Ren YuJun Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Yu Miao Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Ma TengFei Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhang XueLian Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Zhao Jie Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/3(2011-07-01), 551-563 0033-183X 248:3<551 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer