caa a22 4500 445840404 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00709-010-0201-1 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0201-1 Time- and temperature-dependent autolysis of urinary bladder epithelium during ex vivo preservation [Elektronische Daten] [Andreja Erman, Peter Veranič] Morphological and functional preservation of urinary bladder epithelium-urothelium after extirpation from an organism enables physiological studies of that tissue and provides the basis for successful organ transplantations. The aim of this study was to determine the optimal temperature for maintaining urothelium in ex vivo conditions. Mouse urinary bladders were kept at the three temperatures usually used for maintaining tissue during transportation: at the temperature of melting ice (1°C), at room temperature (22-24°C), and at the body temperature of most mammals (37°C). Autolytic structural changes were followed with electron microscopy, while destruction of cytoskeleton and intercellular junctions was observed by immunolabeling. The first ultrastructural changes, swelling of mitochondria and necrosis of individual cells, became evident 30min after extirpation if the tissue was kept at 1°C. After 60 and 120min in ex vivo conditions, the most severe changes with increasing plasma membrane ruptures were detected at 1°C, while at room temperature only mild changes were detected. At 37°C, the extent of ultrastructural changes was between those of the other two experimental temperatures. Autolytic destruction of cytoskeleton and intercellular junctions was not observed before 2h after extirpation. After 4h, severe degradation of cytokeratin 20 and microtubules were found at 1°C and 37°C, while being almost undisturbed at room temperature. On the other hand, the reduction of desmoplakin and ZO-1 labeling was more evident at 37°C than at 1°C and room temperature. These findings provide evidence that room temperature is most appropriate for short ex vivo preservation of urothelial tissue. Springer-Verlag, 2010 Urothelium nationallicence Autolysis nationallicence Tissue preservation nationallicence Ultrastructure nationallicence Cytoskeleton nationallicence Intercellular junctions nationallicence Erman Andreja Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia aut Veranič Peter Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia aut Protoplasma Springer Vienna 248/3(2011-07-01), 541-550 0033-183X 248:3<541 2011 248 709 https://doi.org/10.1007/s00709-010-0201-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0201-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Erman Andreja Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Veranič Peter Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/3(2011-07-01), 541-550 0033-183X 248:3<541 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer