caa a22 4500 445840455 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00709-010-0212-y doi (NATIONALLICENCE)springer-10.1007/s00709-010-0212-y Polysaccharide and glycoprotein distribution in the epidermis of cotton ovules during early fiber initiation and growth [Elektronische Daten] [Andrew Bowling, Kevin Vaughn, Rickie Turley] The cotton fiber is a model system to study cell wall biosynthesis because the fiber cell elongates (∼3cm in ∼20days) without mitosis. In this study, developing cotton ovules, examined from 1day before anthesis (DBA) to 2days post-anthesis (DPA), that would be difficult to investigate via classical carbohydrate biochemistry were probed using a battery of antibodies that recognize a large number of different wall components. In addition, ovules from these same stages were investigated in three fiberless lines. Most antibodies reacted with at least some component of the ovule, and several of the antibodies reacted specifically with the epidermal layer of cells that may give clues as to the nature of the development of the fibers and the neighboring, nonfiber atrichoblasts. Arabinogalactan proteins (AGPs) labeled the epidermal layers more strongly than other ovular tissue, even at 1 DBA. One of the AGP antibodies, CCRC-M7, which recognizes a 1➔6 galactan epitope of AGPs, is lost from the fiber cells by 2 DPA, although labeling in the atrichoblasts remained strong. In contrast, LM5 that recognizes a 1➔4 galactan RGI side chain is unreactive with sections until the fibers are produced and only the fibers are reactive. Dramatic changes also occur in the homogalacturonans (HGs). JIM5, which recognizes highly de-esterified HGs, only weakly labels epidermal cells of 1 DBA and 0 DPA ovules, but labeling increases in fibers cells, where a pectinaceous sheath is produced around the fiber cell and stronger reaction in the internal and external walls of the atrichoblast. In contrast, JIM7-reactive, highly esterifed HGs are present at high levels in the epidermal cells throughout development. Fiberless lines displayed similar patterns of labeling to the fibered lines, except that all of the cells had the labeling pattern of atrichoblasts. That is, CCRC-M7 labeled all cells of the fiberless lines, and LM5 labeled no cells at 2 DPA. These data indicate that a number of polysaccharides are unique in quantity or presence in the epidermal cell layers, and some of these might be critical participants in the early stages of initiation and elongation of cotton fibers. Springer-Verlag (outside the USA), 2010 Cotton nationallicence Fiber initiation nationallicence Pectins nationallicence Arabinogalactan proteins nationallicence Esterification nationallicence AGP : Arabinogalactan protein nationallicence BSA : Bovine serum albumin nationallicence DBA : Days before anthesis nationallicence DPA : Days post-anthesis nationallicence HG : Homogalacturonan nationallicence IGS : Immunogold-silver nationallicence PBS : Phosphate-buffered saline nationallicence RGI : Rhamnogalacturonan I nationallicence Bowling Andrew Crop Production Systems Research Unit, US Department of Agriculture, The Agricultural Research Service (USDA-ARS), 38776, Stoneville, MS, USA aut Vaughn Kevin Crop Production Systems Research Unit, US Department of Agriculture, The Agricultural Research Service (USDA-ARS), 38776, Stoneville, MS, USA aut Turley Rickie Crop Genetics Research Unit, USDA-ARS, PO Box345, 38776, Stoneville, MS, USA aut Protoplasma Springer Vienna 248/3(2011-07-01), 579-590 0033-183X 248:3<579 2011 248 709 https://doi.org/10.1007/s00709-010-0212-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0212-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bowling Andrew Crop Production Systems Research Unit, US Department of Agriculture, The Agricultural Research Service (USDA-ARS), 38776, Stoneville, MS, USA aut NATIONALLICENCE 700 1- Vaughn Kevin Crop Production Systems Research Unit, US Department of Agriculture, The Agricultural Research Service (USDA-ARS), 38776, Stoneville, MS, USA aut NATIONALLICENCE 700 1- Turley Rickie Crop Genetics Research Unit, USDA-ARS, PO Box345, 38776, Stoneville, MS, USA aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/3(2011-07-01), 579-590 0033-183X 248:3<579 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer