caa a22 4500 445840463 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0248-z doi (NATIONALLICENCE)springer-10.1007/s00709-010-0248-z Embryogenesis in Sedum acre L.: structural and immunocytochemical aspects of suspensor development [Elektronische Daten] [Małgorzata Kozieradzka-Kiszkurno, Joanna Świerczyńska, Jerzy Bohdanowicz] The changes in the formation of both the actin and the microtubular cytoskeleton during the differentiation of the embryo-suspensor in Sedum acre were studied in comparison with the development of the embryo-proper. The presence and distribution of the cytoskeletal elements were examined ultrastructurally and with the light microscope using immunolabelling and rhodamine-phalloidin staining. At the globular stage of embryo development extensive array of actin filaments is present in the cytoplasm of basal cell, the microfilament bundles generally run parallel to the long axis of basal cell and pass in close to the nucleus. Microtubules form irregular bundles in the cytoplasm of the basal cell. A strongly fluorescent densely packed microtubules are present in the cytoplasmic layer adjacent to the wall separating the basal cell from the first layer of the chalazal suspensor cells. At the heart-stage of embryo development, in the basal cell, extremely dense arrays of actin materials are located near the micropylar and chalazal end of the cell. At this stage of basal cell formation, numerous actin filaments congregate around the nucleus. In the fully differentiated basal cell and micropylar haustorium, the tubulin cytoskeleton forms a dense prominent network composed of numerous cross-linked filaments. In the distal region of the basal cell, a distinct microtubular cytoskeleton with numerous microtubules is observed in the cytoplasmic layer adjacent to the wall, separating the basal cell from the first layer of the chalazal suspensor cells. The role of cytoskeleton during the development of the suspensor in S. acre is discussed. The Author(s), 2010 Cytoskeleton nationallicence Embryogenesis nationallicence Immunolocalization nationallicence Ultrastructure nationallicence Sedum acre nationallicence Suspensor nationallicence BC : Basal cell nationallicence BSA : Bovine serum albumin nationallicence CHS : Chalazal suspensor cells nationallicence DAPI : 4′ 6′-Diamidino-2-phenylindole dihydrochloride nationallicence DMSO : Dimethyl sulfoxide nationallicence DTT : Dithiothreitol nationallicence EGTA : Ethylene glycol-bis (2-aminoethylether)-N,N,N′,N′-tetraacetic acid nationallicence EP : Embryo-proper nationallicence MH : Micropylar haustorium nationallicence MSB : m-Maleimidobenzoic acid N-hydroxysuccinimide ester nationallicence PBS : Phosphate-buffered saline nationallicence PIPES : Piperazine-N,N′-bis (2-ethanesulfonic acid) nationallicence Kozieradzka-Kiszkurno Małgorzata Department of Plant Cytology and Embryology, University of Gdańsk, Kładki 24 St., 80-822, Gdańsk, Poland aut Świerczyńska Joanna Department of Plant Cytology and Embryology, University of Gdańsk, Kładki 24 St., 80-822, Gdańsk, Poland aut Bohdanowicz Jerzy Department of Plant Cytology and Embryology, University of Gdańsk, Kładki 24 St., 80-822, Gdańsk, Poland aut Protoplasma Springer Vienna 248/4(2011-10-01), 775-784 0033-183X 248:4<775 2011 248 709 https://doi.org/10.1007/s00709-010-0248-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0248-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kozieradzka-Kiszkurno Małgorzata Department of Plant Cytology and Embryology, University of Gdańsk, Kładki 24 St., 80-822, Gdańsk, Poland aut NATIONALLICENCE 700 1- Świerczyńska Joanna Department of Plant Cytology and Embryology, University of Gdańsk, Kładki 24 St., 80-822, Gdańsk, Poland aut NATIONALLICENCE 700 1- Bohdanowicz Jerzy Department of Plant Cytology and Embryology, University of Gdańsk, Kładki 24 St., 80-822, Gdańsk, Poland aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 775-784 0033-183X 248:4<775 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer