caa a22 4500 44584048X CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0249-y doi (NATIONALLICENCE)springer-10.1007/s00709-010-0249-y Metallothionein-like gene from Cicer microphyllum is regulated by multiple abiotic stresses [Elektronische Daten] [Rupesh Singh, Sivalingam Anandhan, Shweta Singh, Vikas Patade, Zakwan Ahmed, Veena Pande] Cicer microphyllum, a wild relative of cultivated chickpea, is a high altitude cold desert-adapted species distributed in western and trans-Himalayas. A complementary DNA (cDNA) encoding metallothionein-like protein has been identified from a cold-induced subtraction cDNA library from C. microphyllum. The sequence of the cloned metallothionein gene from C. microphyllum (GQ900702) contains 240-bp-long open reading frame and encodes predicted 79-amino acid protein of 7.9kDa. Sequence analysis identified the motifs characteristic of type II metallothionein and designated as CmMet-2. Southern hybridization confirms a single copy of the CmMet-2 gene in C. microphyllum genome. In situ hybridization indicated spatial transcript regulation of CmMet-2 in root and aerial parts and also confirmed through real-time PCR-based quantitative transcript analysis. The data revealed a significantly low level of transcript in the aerial parts than the roots. Quantitative analysis using real-time PCR assay revealed induction of transcript in all parts of plants in response to cold stress at 4°C. The transcript abundance was found to increase exponentially with time course from 6 to 24h after exposure. Further, regulation of transcript accumulation in response to abscisic acid application, polyethylene glycol (100μM)-induced osmotic stress, or ZnSO4 (1μM) foliar spray indicated by Northern hybridization suggests the involvement of CmMet-2 in multiple stress response. Springer-Verlag, 2010 Metallothionein nationallicence Cold stress nationallicence ABA nationallicence Dehydration nationallicence Zinc nationallicence Singh Rupesh Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut Anandhan Sivalingam Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut Singh Shweta Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut Patade Vikas Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut Ahmed Zakwan Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut Pande Veena Department of Biotechnology, Kumaon University, Nainital, Uttarakhand, India aut Protoplasma Springer Vienna 248/4(2011-10-01), 839-847 0033-183X 248:4<839 2011 248 709 https://doi.org/10.1007/s00709-010-0249-y text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0249-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Rupesh Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Anandhan Sivalingam Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Singh Shweta Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Patade Vikas Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Ahmed Zakwan Molecular Biology and Genetic Engineering, Defence Institute of Bio Energy Research, Goraparao, Haldwani, 263139, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Pande Veena Department of Biotechnology, Kumaon University, Nainital, Uttarakhand, India aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 839-847 0033-183X 248:4<839 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer