caa a22 4500 44584051X CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0255-0 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0255-0 In silico prediction of drug targets in Vibrio cholerae [Elektronische Daten] [Pramod Katara, Atul Grover, Himani Kuntal, Vinay Sharma] Identification of potential drug targets is the first step in the process of modern drug discovery, subjected to their validation and drug development. Whole genome sequences of a number of organisms allow prediction of potential drug targets using sequence comparison approaches. Here, we present a subtractive approach exploiting the knowledge of global gene expression along with sequence comparisons to predict the potential drug targets more efficiently. Based on the knowledge of 155 known virulence and their coexpressed genes mined from microarray database in the public domain, 357 coexpressed probable virulence genes for Vibrio cholerae were predicted. Based on screening of Database of Essential Genes using blastn, a total of 102 genes out of these 357 were enlisted as vitally essential genes, and hence good putative drug targets. As the effective drug target is a protein which is only present in the pathogen, similarity search of these 102 essential genes against human genome sequence led to subtraction of 66 genes, thus leaving behind a subset of 36 genes whose products have been called as potential drug targets. The gene ontology analysis using Blast2GO of these 36 genes revealed their roles in important metabolic pathways of V. cholerae or on the surface of the pathogen. Thus, we propose that the products of these genes be evaluated as target sites of drugs against V. cholerae in future investigations. Springer-Verlag, 2010 Vibrio cholerae nationallicence Virulence genes nationallicence Essential genes nationallicence Drug targets nationallicence Coexpressed genes nationallicence Katara Pramod Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut Grover Atul Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut Kuntal Himani Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut Sharma Vinay Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut Protoplasma Springer Vienna 248/4(2011-10-01), 799-804 0033-183X 248:4<799 2011 248 709 https://doi.org/10.1007/s00709-010-0255-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0255-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Katara Pramod Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut NATIONALLICENCE 700 1- Grover Atul Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut NATIONALLICENCE 700 1- Kuntal Himani Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut NATIONALLICENCE 700 1- Sharma Vinay Department of Bioscience and Biotechnology, Banasthali University, 304022, Banasthali, India aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 799-804 0033-183X 248:4<799 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer