caa a22 4500 445840528 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0222-9 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0222-9 Gating of aquaporins by heavy metals in Allium cepa L. epidermal cells [Elektronische Daten] [Ewa Przedpelska-Wasowicz, Malgorzata Wierzbicka] Changes in the water permeability, aquaporin (AQP) activity, of leaf cells were investigated in response to different heavy metals (Zn2+, Pb2+, Cd2+, Hg2+). The cell pressure probe experiments were performed on onion epidermal cells as a model system. Heavy metal solutions at different concentrations (0.05μM-2mM) were used in our experiments. We showed that the investigated metal ions can be arranged in order of decreasing toxicity (expressed as a decrease in water permeability) as follows: Hg>Cd>Pb>Zn. Our results showed that β-mercaptoethanol treatment (10mM solution) partially reverses the effect of AQP gating. The magnitude of this reverse differed depending on the metal and its concentration. The time course studies of the process showed that the gating of AQPs occurred within the first 10min after the application of a metal. We also showed that after 20-40min from the onset of metal treatment, the water flow through AQPs stabilized and remained constant. We observed that irrespective of the metal applied, the effect of AQP gating can be recorded within the first 10min after the administration of metal ions. More generally, our results indicate that the toxic effects of investigated metal ions on the cellular level may involve AQP gating. The Author(s), 2010 Epidermal onion cells nationallicence Aquaporins nationallicence Heavy metals nationallicence Cell pressure probe nationallicence Half-time of water exchange nationallicence β-mercaptoethanol nationallicence AQP : Aquaporin nationallicence PPC : Cell pressure probe nationallicence T 1/2 : Half-time of water exchange nationallicence ME : β-mercaptoethanol nationallicence Przedpelska-Wasowicz Ewa Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Wierzbicka Malgorzata Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut Protoplasma Springer Vienna 248/4(2011-10-01), 663-671 0033-183X 248:4<663 2011 248 709 https://doi.org/10.1007/s00709-010-0222-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0222-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Przedpelska-Wasowicz Ewa Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 700 1- Wierzbicka Malgorzata Department of Molecular Plant Physiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 663-671 0033-183X 248:4<663 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer