caa a22 4500 445840552 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0257-y doi (NATIONALLICENCE)springer-10.1007/s00709-010-0257-y Differential antioxidative responses to dehydration-induced oxidative stress in core set of foxtail millet cultivars [ Setaria italica (L.)] [Elektronische Daten] [Charu Lata, Sarita Jha, Vivek Dixit, Nese Sreenivasulu, Manoj Prasad] Foxtail millet (Setaria italica L.) known as a relatively drought-tolerant crop across the world is grown in arid and semi-arid regions. To the best of our knowledge, no systematic study on drought tolerance screening of foxtail millet germplasm being a drought-tolerant crop has been reported so far. To explore genetic diversity of drought-induced oxidative stress tolerance in foxtail millet, we employed lipid peroxidation measure to assess membrane integrity under stress as biochemical marker to screen 107 cultivars and classified the genotypes as highly tolerant, tolerant, sensitive, and highly sensitive. From this comprehensive screening, four cultivars showing differential response to dehydration tolerance were selected to understand the physiological and biochemical basis of tolerance mechanisms. The dehydration-tolerant cultivars (IC-403579 and Prasad) showed considerably lower levels of lipid peroxidation and electrolyte leakage as compared with dehydration-sensitive cultivars (IC-480117 and Lepakshi), indicating better cell membrane integrity in tolerant cultivars. Correspondingly, tolerant genotypes maintained higher activity of catalase (EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), and glutathione reductase (GR; EC 1.6.4.2) across different time-course period of polyethylene glycol (PEG) treatments in comparison to sensitive ones. The above biochemical results were further validated through quantitative real-time PCR analysis of APX and GR, whose transcripts were substantially induced by PEG treatments in tolerant cultivars. These results suggest that tolerant cultivars possess wider array of antioxidant machinery with efficient ascorbate-glutathione pathway to cope with drought-induced oxidative stress. Springer-Verlag, 2010 Setaria italica nationallicence Dehydration nationallicence Relative water content (RWC) nationallicence Lipid peroxidation (LP) nationallicence Electrolytic leakage (EL) nationallicence Reactive oxygen species (ROS) nationallicence Antioxidative enzymes nationallicence Quantitative real-time PCR (qRT-PCR) nationallicence Lata Charu National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut Jha Sarita National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut Dixit Vivek National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut Sreenivasulu Nese Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 03, 06466, Gatersleben, Germany aut Prasad Manoj National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut Protoplasma Springer Vienna 248/4(2011-10-01), 817-828 0033-183X 248:4<817 2011 248 709 https://doi.org/10.1007/s00709-010-0257-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0257-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lata Charu National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Jha Sarita National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Dixit Vivek National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Sreenivasulu Nese Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 03, 06466, Gatersleben, Germany aut NATIONALLICENCE 700 1- Prasad Manoj National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, JNU Campus, 110067, New Delhi, India aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 817-828 0033-183X 248:4<817 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer