caa a22 4500 445840579 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0229-2 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0229-2 Laticiferous canal formation in fruits of Decaisnea fargesii [Elektronische Daten] a programmed cell death process? [Ya-Fu Zhou, Wen-Zhe Liu] Programmed cell death (PCD), a topic of abiding interest, remodels plants at the cell, tissue, and organ levels involving various developmental processes of plants. The aim of this study is to provide a morphological characterization of evidence of PCD involvement in the laticiferous canal formation in fruit of Decaisnea fargesii. Several ultrastructural features of PCD have been observed including disintegration of vacuole and plasma membranes, cell wall degeneration, degenerated cytoplasm, abundant membrane structures and flocculent material, mitochondria and misshapen nuclei coupled with degraded plastids in vacuoles, and nuclei enveloped by rubber granule. In D. fargesii, the nuclei of the secretory epidermal cells become TUNEL-positive from the sunken stage to the late expanding stage, then DAPI-negative during the mature stage, indicating an early event of deoxyribonucleic acid (DNA) cleavage and a late event of complete DNA degeneration. Gel electrophoresis indicates that DNA cleavage is random and does not result in the laddering pattern indicating multiples of internucleosomal units. During the PCD of secretory epidermal cells, the rubber granules continue to be synthesized and accumulated in the secretory epidermal cells despite nuclear degradation. The PCD's role in laticiferous canal formation suggests that PCD may play important roles in gland development of plants. Springer-Verlag, 2010 Laticiferous canal nationallicence Programmed cell death (PCD) nationallicence Autophagy nationallicence TUNEL assay nationallicence DAPI assay nationallicence Decaisnea fargesii Franch nationallicence PCD : programmed cell death nationallicence TUNEL : terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling nationallicence Zhou Ya-Fu Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibai Bei Road, 710069, Xi'an, China aut Liu Wen-Zhe Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibai Bei Road, 710069, Xi'an, China aut Protoplasma Springer Vienna 248/4(2011-10-01), 683-694 0033-183X 248:4<683 2011 248 709 https://doi.org/10.1007/s00709-010-0229-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0229-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Ya-Fu Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibai Bei Road, 710069, Xi'an, China aut NATIONALLICENCE 700 1- Liu Wen-Zhe Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life Science, Northwest University, 229 Taibai Bei Road, 710069, Xi'an, China aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 683-694 0033-183X 248:4<683 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer