caa a22 4500 445840587 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0242-5 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0242-5 Development of the cotyledon cells during olive ( Olea europaea L.) in vitro seed germination and seedling growth [Elektronische Daten] [Agnieszka Zienkiewicz, José Jiménez-López, Krzysztof Zienkiewicz, Juan de Dios Alché, María Rodríguez-García] The structural changes occurred in differentiating olive cotyledon cells into mesophyll cells are described. Using histological and immunocytological methods as well as microscopic observations, we showed that in the cells of mature embryo, large electron-dense proteins bodies (PBs) are surrounded by numerous oil bodies (OBs). After 3days of in vitro germination, the presence of large PBs originated by fusion of smaller PBs was observed. It was also detected a close spatial proximity between PBs and OBs, likely as a reflection of interconnected metabolic pathways. Between the 3rd and the 12th day of germination, the formation of a large vacuolar compartment takes place accompanied by a decrease in the PBs and OBs number. This was coincident with a progressive decrease in the amount of the 11S-type seed storage proteins (SSPs), showed in situ and after Western blot analysis of crude protein extracts. After 26days germination, the cellular organization became typical for a leaf mesophyll cell, with well-differentiated chloroplasts surrounding a large central vacuole. Our results suggest that the olive cotyledon storage reserves are mobilized gradually until the seedling becomes autotrophic. Moreover, the specific accumulation of storage proteins in the intravacuolar material suggests that these structures may operate as a shuttle for SSPs and/or products of their degradation into the cytoplasm, where finally they supply amino acids for the differentiating mesophyll cells. Springer-Verlag, 2010 Cotyledon cells nationallicence In vitro germination nationallicence Olea europaea L nationallicence Oil bodies nationallicence Protein bodies nationallicence Seed storage proteins (SSPs) nationallicence Seed nationallicence Zienkiewicz Agnieszka Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut Jiménez-López José Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut Zienkiewicz Krzysztof Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut de Dios Alché Juan Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut Rodríguez-García María Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut Protoplasma Springer Vienna 248/4(2011-10-01), 751-765 0033-183X 248:4<751 2011 248 709 https://doi.org/10.1007/s00709-010-0242-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0242-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zienkiewicz Agnieszka Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut NATIONALLICENCE 700 1- Jiménez-López José Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut NATIONALLICENCE 700 1- Zienkiewicz Krzysztof Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut NATIONALLICENCE 700 1- de Dios Alché Juan Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut NATIONALLICENCE 700 1- Rodríguez-García María Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Profesor Albareda 1, 18008, Granada, Spain aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 751-765 0033-183X 248:4<751 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer