caa a22 4500 445840595 CHVBK 20180317145342.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0231-8 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0231-8 Pectin dynamic and distribution of exchangeable Ca2+ in Haemanthus albiflos hollow style during pollen-pistil interactions [Elektronische Daten] [Marta Lenartowska, Magdalena Krzesłowska, Elżbieta Bednarska] In this report, the localization and spatial distribution of two categories of pectin, high and low methylesterified, on the background of dynamic in loosely bound calcium (Ca2+) in Haemanthus hollow style were studied before and after pollination. In the style transmitting tract of unpollinated pistil, mainly high-methylesterified pectins were present, both in the transmitting tract epidermis and in the style canal. After pollination, an increase in the level of two investigated categories of pectin was observed, but the amount of high-methylesterified one in each period of time analyzed was permanently higher. Locally, in the regions of the style canal penetrated by pollen tubes, process of pectin de-esterification was initiated. However, pollination caused an increase of loosely bound Ca2+ level in the style transmitting tract, this process appears to be not linked with pectin de-esterification and possible Ca2+ release after the lysis of Ca2+ cross-linked de-esterified pectin. Instead, it seems to be based on Ca2+ exocytosis from the transmitting tract epidermis cells providing a source of Ca2+ for pollen tubes growing in Haemanthus hollow style. Springer-Verlag, 2010 Ca2+ nationallicence Haemanthus albiflos nationallicence Hollow style nationallicence Pectin nationallicence Pollen tube nationallicence Transmitting tract nationallicence BSA : bovine serum albumin nationallicence Ca2+ : calcium nationallicence CRT : calreticulin nationallicence ER : endoplasmic reticulum nationallicence ecm : extracellular matrix nationallicence HGs : homogalactouronan nationallicence MAb JIM7 : monoclonal antibody against high-methylesterified homogalacturonic acid nationallicence MAb JIM5 : monoclonal antibody against low-methylesterified homogalacturonic acid nationallicence PBS : phosphate-buffered saline nationallicence ppts : Ca2+ precipitates nationallicence stt : style transmitting tract nationallicence tte : transmitting tract epidermis nationallicence Lenartowska Marta Laboratory of Developmental Biology, Faculty of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland aut Krzesłowska Magdalena Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland aut Bednarska Elżbieta Department of Cell Biology, Faculty of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland aut Protoplasma Springer Vienna 248/4(2011-10-01), 695-705 0033-183X 248:4<695 2011 248 709 https://doi.org/10.1007/s00709-010-0231-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0231-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lenartowska Marta Laboratory of Developmental Biology, Faculty of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland aut NATIONALLICENCE 700 1- Krzesłowska Magdalena Laboratory of General Botany, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznań, Poland aut NATIONALLICENCE 700 1- Bednarska Elżbieta Department of Cell Biology, Faculty of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100, Toruń, Poland aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 695-705 0033-183X 248:4<695 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer