caa a22 4500 445840633 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0254-1 doi (NATIONALLICENCE)springer-10.1007/s00709-010-0254-1 Rac-dependent doubling of HeLa cell area and impairment of cell migration and cell cycle by compounds from Iris germanica [Elektronische Daten] [Michal Halpert, Mohamad Abu-Abied, Dror Avisar, Yoni Moskovitz, Osnat Altshuler, Avner Cohen, Mira Weissberg, Joseph Riov, Hugo Gottlieb, Avichai Perl, Einat Sadot] Plants are an infinite source of bioactive compounds. We screened the Israeli flora for compounds that interfere with the organization of the actin cytoskeleton. We found an activity in lipidic extract from Iris germanica that was able to increase HeLa cell area and adhesion and augment the formation of actin stress fibers. This effect was not observed when Ref52 fibroblasts were tested and was not the result of disruption of microtubules. Further, the increase in cell area was Rac1-dependent, and the iris extract led to slight Rac activation. Inhibitor of RhoA kinase did not interfere with the ability of the iris extract to increase HeLa cell area. The increase in HeLa cell area in the presence of iris extract was accompanied by impairment of cell migration and arrest of the cell cycle at G1 although the involvement of Rac1 in these processes is not clear. Biochemical verification of the extract based on activity-mediated fractionation and nuclear magnetic resonance analysis revealed that the active compounds belong to the group of iridals, a known group of triterpenoid. Purified iripallidal was able to increase cell area of both HeLa and SW480 cells. Springer-Verlag, 2011 Iris germanica nationallicence Iridals nationallicence Iripallidal nationallicence Actin nationallicence HeLa cells nationallicence Cell spreading nationallicence DIC : Differential interference contrast nationallicence FACS : Fluorescence-activated cell sorting nationallicence PKC : Protein kinase C nationallicence GAP : GTPase-activating protein nationallicence ECM : Extracellular matrix nationallicence TLC : Thin layer chromatography nationallicence DN : Dominant negative nationallicence CA : Constitutive active nationallicence PI : Propidium iodide nationallicence Halpert Michal The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Abu-Abied Mohamad The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Avisar Dror The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Moskovitz Yoni The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Altshuler Osnat The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Cohen Avner The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Weissberg Mira The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Riov Joseph The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut Gottlieb Hugo Department of Chemistry, Bar-Ilan University, 52900, Ramat-Gan, Israel aut Perl Avichai The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Sadot Einat The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut Protoplasma Springer Vienna 248/4(2011-10-01), 785-797 0033-183X 248:4<785 2011 248 709 https://doi.org/10.1007/s00709-010-0254-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0254-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Halpert Michal The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Abu-Abied Mohamad The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Avisar Dror The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Moskovitz Yoni The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Altshuler Osnat The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Cohen Avner The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Weissberg Mira The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Riov Joseph The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100, Rehovot, Israel aut NATIONALLICENCE 700 1- Gottlieb Hugo Department of Chemistry, Bar-Ilan University, 52900, Ramat-Gan, Israel aut NATIONALLICENCE 700 1- Perl Avichai The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 700 1- Sadot Einat The Institute of Plant Sciences, Volcani Center, P.O. Box6, 50250, Bet-Dagan, Israel aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 785-797 0033-183X 248:4<785 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer