caa a22 4500 44584065X CHVBK 20180317145343.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00709-010-0256-z doi (NATIONALLICENCE)springer-10.1007/s00709-010-0256-z Redox state and energetic equilibrium determine the magnitude of stress in Hydrilla verticillata upon exposure to arsenate [Elektronische Daten] [Sudhakar Srivastava, Penna Suprasanna, Stanislaus D'Souza] Arsenic (As) is a potential hazard to plants' health, however the mechanisms of its toxicity are yet to be properly understood. To determine the impact of redox state and energetic in stress imposition, plants of Hydrilla verticillata (L.f.) Royle, which are known to be potential accumulator of As, were exposed to 100 and 500μM arsenate (AsV) for 4 to 96h. Plants demonstrated significant As accumulation with the maximum being at 500μM after 96h (568μgg−1dry weight, dw). The accumulation of As led to a significant increase in the level of reactive oxygen species, nitric oxide, carbonyl, malondialdehyde, and percentage of DNA degradation. In addition, the activity of pro-oxidant enzymes like NADPH oxidase and ascorbate oxidase also showed significant increases. These parameters collectively indicated oxidative stress, which in turn caused an increase in percentage of cell death. These negative effects were seemingly linked to an altered energetic and redox equilibrium [analyzed in terms of ATP/ADP, NADH/NAD, NADPH/NADP, reduced glutathione/oxidized glutathione, and ascorbate/dehydroascobate ratios]. Although there was significant increase in the levels of phytochelatins, the As chelating ligands, a large amount of As was presumably present as free ion particularly at 500μM AsV, which supposedly produced toxic responses. In conclusion, the study demonstrated that the magnitude of disturbance to redox and energetic equilibrium of plants upon AsV exposure determines the extent of toxicity to plants. Springer-Verlag, 2010 Arsenic toxicity nationallicence Energetic nationallicence Hydrilla verticillata nationallicence Oxidative stress nationallicence Redox state nationallicence AO : Ascorbate oxidase nationallicence ASC : Reduced ascorbate nationallicence DHA : Dehydroascorbate nationallicence GSH : Reduced glutathione nationallicence GSSG : Oxidized glutathione nationallicence NAD : Nicotinamide adenine dinucleotide nationallicence NADH : Nicotinamide adenine dinucleotide reduced nationallicence NADP : Nicotinamide adenine dinucleotide phosphate nationallicence NADPH : Nicotinamide adenine dinucleotide phosphate reduced nationallicence NO : Nitric oxide nationallicence ROS : Reactive oxygen species nationallicence Srivastava Sudhakar Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Mumbai, India aut Suprasanna Penna Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Mumbai, India aut D'Souza Stanislaus Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Mumbai, India aut Protoplasma Springer Vienna 248/4(2011-10-01), 805-815 0033-183X 248:4<805 2011 248 709 https://doi.org/10.1007/s00709-010-0256-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0256-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Srivastava Sudhakar Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Mumbai, India aut NATIONALLICENCE 700 1- Suprasanna Penna Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Mumbai, India aut NATIONALLICENCE 700 1- D'Souza Stanislaus Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, 400085, Mumbai, India aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/4(2011-10-01), 805-815 0033-183X 248:4<805 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer