caa a22 4500 445840676 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0176-y doi (NATIONALLICENCE)springer-10.1007/s00709-010-0176-y Pollen tube reuses intracellular components of nucellar cells undergoing programmed cell death in Pinus densiflora [Elektronische Daten] [Rie Hiratsuka, Osamu Terasaka] Through the process known as programmed cell death (PCD), nucelli of Pinus densiflora serve as the transmitting tissue for growth of the pollen tube. We sought to clarify the processes of degradation of nucellar cell components and their transport to the pollen tube during PCD in response to pollen tube penetration of such nucelli. Stimulated by pollination, synthesis of large amounts of starch grains occurred in cells in a wide region of the nucellus, but as the pollen tube penetrated the nucellus, starch grains were degraded in amyloplasts of nucellar cells. In cells undergoing PCD, electron-dense vacuoles with high membrane contrast appeared, assumed a variety of autophagic structures, expanded, and ultimately collapsed and disappeared. Vesicles and electron-dense amorphous materials were released inside the thickened walls of cells undergoing PCD, and those vesicles and materials reaching the pollen tube after passing through the extracellular matrix were taken into the tube by endocytosis. These results show that in PCD of nucellar cells, intracellular materials are degraded in amyloplasts and vacuoles, and some of the degraded material is supplied to the pollen tube by vesicular transport to support tube growth. Springer-Verlag, 2010 Gymnosperm nationallicence Nucellus nationallicence Pinus densiflora nationallicence Pollen tube nationallicence Programmed cell death nationallicence Hiratsuka Rie Division of Biology, Department of Natural Science, Jikei University School of Medicine, 8-3-1, Kokuryo, 182-8570, Chofu- city, Tokyo, Japan aut Terasaka Osamu Division of Biology, Department of Natural Science, Jikei University School of Medicine, 8-3-1, Kokuryo, 182-8570, Chofu- city, Tokyo, Japan aut Protoplasma Springer Vienna 248/2(2011-04-01), 339-351 0033-183X 248:2<339 2011 248 709 https://doi.org/10.1007/s00709-010-0176-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0176-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hiratsuka Rie Division of Biology, Department of Natural Science, Jikei University School of Medicine, 8-3-1, Kokuryo, 182-8570, Chofu- city, Tokyo, Japan aut NATIONALLICENCE 700 1- Terasaka Osamu Division of Biology, Department of Natural Science, Jikei University School of Medicine, 8-3-1, Kokuryo, 182-8570, Chofu- city, Tokyo, Japan aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 339-351 0033-183X 248:2<339 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer