caa a22 4500 445840692 CHVBK 20180317145343.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00709-010-0175-z doi (NATIONALLICENCE)springer-10.1007/s00709-010-0175-z Endosymbiosis of Chlorella species to the ciliate Paramecium bursaria alters the distribution of the host's trichocysts beneath the host cell cortex [Elektronische Daten] [Yuuki Kodama, Masahiro Fujishima] Each symbiotic Chlorella of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole membrane derived from the host digestive vacuole membrane. Alga-free paramecia and symbiotic algae can grow independently. Mixing them experimentally can cause reinfection. Earlier, we reported that the symbiotic algae appear to push the host trichocysts aside to become fixed beneath the host cell cortex during the algal reinfection process. Indirect immunofluorescence microscopy with a monoclonal antibody against the trichocysts demonstrates that the trichocysts change their locality to form algal attachment sites and decrease their density beneath the host cell cortex through algal reinfection. Transmission electron microscopy to detect acid phosphatase activity showed that some trichocysts near the host cell cortex are digested by the host lysosomal fusion during algal reinfection. Removal of algae from the host cell using cycloheximide recovers the trichocyst's arrangement and number near the host cell cortex. These results indicate that symbiotic algae compete for their attachment sites with preexisting trichocysts and that the algae have the ability to ensure algal attachment sites beneath the host cell cortex. Springer-Verlag, 2010 Acid phosphatase activity nationallicence Cell cortex nationallicence Endosymbiosis nationallicence Monoclonal antibody nationallicence Perialgal vacuole membrane nationallicence Trichocysts nationallicence Kodama Yuuki Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yoshida 1677-1, 753-8512, Yamaguchi, Japan aut Fujishima Masahiro Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yoshida 1677-1, 753-8512, Yamaguchi, Japan aut Protoplasma Springer Vienna 248/2(2011-04-01), 325-337 0033-183X 248:2<325 2011 248 709 https://doi.org/10.1007/s00709-010-0175-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00709-010-0175-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kodama Yuuki Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yoshida 1677-1, 753-8512, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Fujishima Masahiro Department of Environmental Science and Engineering, Graduate School of Science and Engineering, Yamaguchi University, Yoshida 1677-1, 753-8512, Yamaguchi, Japan aut NATIONALLICENCE 773 0- Protoplasma Springer Vienna 248/2(2011-04-01), 325-337 0033-183X 248:2<325 2011 248 709 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer